Mbuh J V, Fagbemi B O
Department of Veterinary Microbiology and Parasitology, University of Ibadan, Nigeria.
Vet Parasitol. 1996 Nov 15;66(3-4):171-9. doi: 10.1016/s0304-4017(96)01019-9.
The profiles of antibody response and circulating antigen levels in goats infected with Fasciola gigantica were studied by enzyme-linked immunoelectrotransfer blot (EITB) and enzyme-linked immunosorbent assay (ELISA). In the antibody assay, sera from goats experimentally infected with F. gigantica were reacted with whole worm antigen of the worm before and after chemotherapy with oxyclozanide. In ELISA, there was a significant increase in antibody level 2 weeks after infection. After chemotherapy, there was a gradual decrease in antibody within 3 weeks followed by a rapid decline by the 4th week after treatment. By EITB, the infected goat sera recognized three polypeptides in the range of 42-80 kDa as early as 2 weeks after infection. Recognition of the complete components of F. gigantica antigen repertoire occurred as early as the 4th week after infection. By the 8th week after chemotherapy, distinct polypeptide band recognition was no longer possible. Comparative immunoblotting with goat anti-Paramphistomum, anti-Dicrocoelium and anti-Fasciola sera revealed that the 14 kDa, 17 kDa, 21 kDa, 28 kDa and 30 kDa proteins are specific to F. gigantica. In the antigen assay, circulating antigen was detectable by the direct ELISA method one week after infection and negative absorbance values were observed 4 weeks after chemotherapy.
通过酶联免疫电转移印迹法（EITB）和酶联免疫吸附测定法（ELISA）研究了感染巨片形吸虫的山羊的抗体反应和循环抗原水平。在抗体检测中，将经实验感染巨片形吸虫的山羊血清与在使用羟氯扎胺化疗前后的虫体全虫抗原进行反应。在ELISA检测中，感染后2周抗体水平显著升高。化疗后，抗体在3周内逐渐下降，随后在治疗后第4周迅速下降。通过EITB检测，感染山羊血清早在感染后2周就能识别42 - 80 kDa范围内的三种多肽。早在感染后第4周就能识别巨片形吸虫抗原库的完整成分。化疗后第8周，不再能识别出明显的多肽条带。用山羊抗双口吸虫、抗片形吸虫和抗巨片形吸虫血清进行的比较免疫印迹显示，14 kDa、17 kDa、21 kDa、28 kDa和30 kDa的蛋白质是巨片形吸虫特有的。在抗原检测中，感染后1周通过直接ELISA法可检测到循环抗原，化疗后4周观察到阴性吸光度值。
