Guobadia E E, Fagbemi B O
Department of Veterinary Microbiology and Parasitology, University of Ibadan, Nigeria.
Vet Parasitol. 1997 Feb;68(3):269-82. doi: 10.1016/s0304-4017(96)01065-5.
The 17kDa and 69kDa polypeptide antigens which are specific to Fasciola gigantica were excised from polyacrylamide gels and used for the immunization of rabbits to raise monospecific antisera against these polypeptides. These sera were labelled with horseradish peroxidase and used for the detection of circulating 17 kDa and 69 kDa antigens by a direct enzyme-linked immunosorbent assay in the sera of sheep that were experimentally or naturally infected with F. gigantica. The 17 kDa antigen was detected in the sera of infected sheep as early as 1 week after infection and, following chemotherapy with oxyclozanide, negative seroconversion occurred 2 weeks later. The 69 kDa antigen was detectable as from the fourth week of infection and its detection ceased 3 weeks after chemotherapy. The serodiagnosis of F. gigantica, based on the detection of the 17 kDa antigen in sheep sera, was more specific and sensitive than that based on the detection of the 69 kDa antigen.
从聚丙烯酰胺凝胶中切下对巨片形吸虫具有特异性的17kDa和69kDa多肽抗原，用于免疫兔子以产生针对这些多肽的单特异性抗血清。这些血清用辣根过氧化物酶标记，并通过直接酶联免疫吸附测定法用于检测实验性或自然感染巨片形吸虫的绵羊血清中循环的17kDa和69kDa抗原。感染后1周即可在感染绵羊的血清中检测到17kDa抗原，在用羟氯扎胺化疗后，2周后出现血清学转阴。69kDa抗原从感染第4周开始可检测到，化疗3周后检测不到。基于检测绵羊血清中17kDa抗原的巨片形吸虫血清学诊断比基于检测69kDa抗原的诊断更具特异性和敏感性。
