Labbé R G, Chang C A
Department of Food Science, University of Massachusetts-Amherst 01003, USA.
Lett Appl Microbiol. 1995 Nov;21(5):302-6. doi: 10.1111/j.1472-765x.1995.tb01065.x.
Heat-injured spores of several strains of Clostridium perfringens types B, C and D could be partially recovered if lysozyme was included in the recovery medium. As little as 25 ng ml-1 was effective. D90 degrees C values of 1.3-2.6 were obtained with an approximate 2-3-fold increase in the presence of 1 /microgram ml-1 of lysozyme. In the absence of lysozyme, prolonged heating of spores resulted in the appearance of satellite colonies surrounding colonies of surviving spores. An initiation protein, previously reported in the case of type A strains, was also produced by type B, C and D strains. When added to the recovery medium it too promoted the recovery of spores from thermal injury though not as effectively as lysozyme.
如果在复苏培养基中加入溶菌酶，几种B型、C型和D型产气荚膜梭菌的热损伤孢子能够部分复苏。低至25纳克/毫升就有效。在1微克/毫升溶菌酶存在的情况下，90摄氏度时的D值为1.3 - 2.6，孢子复苏率大约提高了2至3倍。在没有溶菌酶的情况下，孢子长时间加热会导致在存活孢子菌落周围出现卫星菌落。先前在A型菌株中报道的一种起始蛋白，B型、C型和D型菌株也能产生。当添加到复苏培养基中时，它也能促进热损伤孢子的复苏，不过效果不如溶菌酶。
