Yoon K J, Zimmerman J J, Swenson S L, McGinley M J, Eernisse K A, Brevik A, Rhinehart L L, Frey M L, Hill H T, Platt K B
Department of Microbiology, Immunology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
J Vet Diagn Invest. 1995 Jul;7(3):305-12. doi: 10.1177/104063879500700302.
The development of the humoral immune response against porcine reproductive and respiratory syndrome (PRRS) virus was monitored by an indirect fluorescent antibody (IFA) test, immunoperoxidase monolayer assay (IPMA), enzyme-linked immunosorbent assay (ELISA), and serum virus neutralization (SVN) test over a 105-day period in 8 pigs experimentally infected with ATCC strain VR-2402. Specific antibodies against PRRS virus were first detected by the IFA test, IPMA, ELISA, and the SVN test 9-11, 5-9, 9-13, and 9-28 days postinoculation (PI), respectively, and reached their maximum values by 4-5, 5-6, 4-6, and 10-11 weeks PI, respectively, thereafter. After reaching maximum value, all assays showed a decline in antibody levels. Assuming a constant rate of antibody decay, it was estimated by regression analysis that the ELISA, IFA, IPMA, and SVN antibody titers would approach the lower limits of detection by approximately days 137, 158, 324, and 356 PI, respectively. In this study, the immunoperoxidase monolayer assay appeared to offer slightly better performance relative to the IFA test, ELISA, and SVN test in terms of earlier detection and slower rate of decline in antibody titers. Western immunoblot analysis revealed that antibody specific for the 15-kD viral protein was present in all pigs by 7 days PI and persisted throughout the 105-day observation period. Initial detection of antibodies to the 19-, 23-, and 26-kD proteins varied among pigs, ranging from 9 to 35 days PI. Thereafter, the antibody responses to these 3 viral proteins of PRRS virus continued to be detected throughout the 105-day study period.(ABSTRACT TRUNCATED AT 250 WORDS)
在8头经实验感染ATCC株VR - 2402的猪中，通过间接荧光抗体（IFA）试验、免疫过氧化物酶单层试验（IPMA）、酶联免疫吸附试验（ELISA）和血清病毒中和（SVN）试验，在105天的时间里监测了针对猪繁殖与呼吸综合征（PRRS）病毒的体液免疫反应的发展情况。分别在接种后（PI）9 - 11天、5 - 9天、9 - 13天和9 - 28天，通过IFA试验、IPMA、ELISA和SVN试验首次检测到针对PRRS病毒的特异性抗体，之后分别在PI 4 - 5周、5 - 6周、4 - 6周和10 - 11周达到最大值。达到最大值后，所有检测方法均显示抗体水平下降。假设抗体衰减速率恒定，通过回归分析估计，ELISA、IFA、IPMA和SVN抗体滴度分别在PI约137天、158天、324天和356天接近检测下限。在本研究中，免疫过氧化物酶单层试验在抗体滴度的早期检测和下降速率较慢方面，相对于IFA试验、ELISA和SVN试验似乎表现稍好。蛋白质免疫印迹分析显示，到PI 7天时，所有猪中均存在针对15-kD病毒蛋白的抗体，并且在整个105天的观察期内持续存在。针对19-kD、23-kD和26-kD蛋白的抗体的初始检测在猪之间有所不同，范围为PI 9至35天。此后，在整个105天的研究期间持续检测到对PRRS病毒这3种病毒蛋白的抗体反应。（摘要截短于250字）
