Libersky E A, Boatman D E
Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison 53706, USA.
Biol Reprod. 1995 Sep;53(3):477-82. doi: 10.1095/biolreprod53.3.477.
In vitro, progesterone induces capacitation, hyperactivated motility, and acrosome reactions in sperm, possibly acting through a non-genomic plasma membrane receptor, but neither progesterone functions nor concentration within the mammalian oviduct during the periovulatory period is well characterized. The objectives of this study were to determine the physiological concentrations of progesterone in serum, follicular fluid, and oviductal fluid of golden hamsters during the period of the reproductive cycle in which capacitation and fertilization occur. Fluids were collected from different groups of animals for each of four time points: the normal time for mating (5-6 h post-LH surge [post-LH]), early in capacitation (8-9 h post-LH), immediately preovulation (11-12 h post-LH), and after ovulation near the beginning of fertilization (14-15 h post-LH). Oviductal fluid was collected by 1-h cannulation followed by cardiac puncture for serum collection and follicle aspiration for follicular fluid. Within each time period, the three types of fluid differed significantly in progesterone concentration. Over time, concentrations of progesterone did not change in either serum (range: 5.64-12.85 ng/ml) or follicular fluid (range: 4.2-7.4 micrograms/ml), but the concentration of progesterone in oviductal fluid decreased from 175.06 ng/ml at the first period to 44.01 ng/ml at the fourth (p < 0.05), while the volume of oviductal secretions collected by the same sampling procedure increased from 1.6 to 2.9 microliters (p < 0.05). With this information concerning in vivo concentrations of progesterone during capacitation and fertilization, the physiological role of progesterone in sperm-egg interactions can be addressed.
在体外，孕酮可诱导精子获能、超活化运动及顶体反应，可能是通过一种非基因组的质膜受体发挥作用，但在围排卵期哺乳动物输卵管内，孕酮的功能及浓度均未得到充分表征。本研究的目的是确定金黄仓鼠在生殖周期中发生精子获能和受精的时期，其血清、卵泡液及输卵管液中孕酮的生理浓度。在四个时间点分别从不同组动物收集液体：正常交配时间（促黄体生成素高峰后5 - 6小时[LH后]）、获能早期（LH后8 - 9小时）、排卵前即刻（LH后11 - 12小时）以及排卵后受精开始时附近（LH后14 - 15小时）。通过1小时插管收集输卵管液，随后通过心脏穿刺收集血清，通过卵泡抽吸收集卵泡液。在每个时间段内，三种液体的孕酮浓度差异显著。随着时间推移，血清（范围：5.64 - 12.85纳克/毫升）和卵泡液（范围：4.2 - 7.4微克/毫升）中的孕酮浓度均未发生变化，但输卵管液中的孕酮浓度从第一个时间段的175.06纳克/毫升降至第四个时间段的44.01纳克/毫升（p < 0.05），而通过相同采样程序收集的输卵管分泌物量从1.6微升增加至2.9微升（p < 0.05）。有了关于精子获能和受精期间孕酮体内浓度的这些信息，就可以探讨孕酮在精卵相互作用中的生理作用。
