Cascante M, Curto R, Sorribas A
Departament de Bioquímica i Fisiología, Facultat de Químiques, Universitat de Barcelona, Spain.
Math Biosci. 1995 Nov;130(1):51-69. doi: 10.1016/0025-5564(94)00093-f.
In the preceding paper in this issue, we have shown that metabolic control analysis and biochemical systems theory use the same experimental information to describe a metabolic system. In this paper, we analyze the steady-state properties of this pathway by applying both methods. Our results show the correspondence of the steady-state characterizations and illustrate the relationships between the different nomenclatures used. With both approaches, we identify metabolite pools that are strongly influenced by changes in enzyme concentration when cells are immobilized at pH 5.5. In the final paper of this series, which follows, we discuss the need to assess the quality of a model and the potential difficulties that may arise if the steady-state characterization is accepted without testing its quality. We then validate the different models using parameter sensitivity concepts.
在本期的上一篇论文中，我们已经表明代谢控制分析和生化系统理论使用相同的实验信息来描述一个代谢系统。在本文中，我们通过应用这两种方法来分析该途径的稳态特性。我们的结果显示了稳态特征的对应关系，并说明了所使用的不同命名法之间的关系。通过这两种方法，我们确定了在细胞固定于pH 5.5时，哪些代谢物池会受到酶浓度变化的强烈影响。在本系列接下来的最后一篇论文中，我们将讨论评估模型质量的必要性，以及如果在未经质量测试就接受稳态特征可能出现的潜在困难。然后，我们使用参数敏感性概念来验证不同的模型。
