Kesner J S, Knecht E A, Krieg E F
Experimental Toxicology Branch, National Institute for Occupational Safety and Health, Cincinnati, OH 45226-1998, USA.
Reprod Toxicol. 1995 May-Jun;9(3):239-44. doi: 10.1016/0890-6238(95)00005-u.
Urinary reproductive hormones afford specific and sensitive evaluation of female reproductive potential in epidemiologic and clinical settings. The goal of this study was to characterize the stability of urinary luteinizing hormone, follicle stimulating hormone, estrone 3-glucuronide, pregnanediol 3-glucuronide, and creatinine during storage as functions of time, temperature, and additives. After 2 weeks with no additives, activity of the four analytes, relative to initial concentrations, ranged from 91.9 to 102.8% at 4 degrees C, 35.1 to 89.6% at 25 degrees C, and 7.5 to 66.9% at 37 degrees C. Antimicrobial additives did not consistently improve stability. Analyte activity for samples stored with no additives for 24 weeks at -80 degrees C ranged from 69.0 to 101.2%. Glycerol and bovine serum albumin improved analyte stability; activity ranged from 91.1 to 106.3%. Other additives were ineffective. These results reveal conditions for storing reproductive hormone analytes in urine during epidemiologic field studies.
泌尿生殖激素在流行病学和临床环境中为评估女性生殖潜力提供了特异且灵敏的指标。本研究的目的是确定尿促黄体生成素、卵泡刺激素、雌酮3 - 葡萄糖醛酸苷、孕二醇3 - 葡萄糖醛酸苷和肌酐在储存过程中随时间、温度和添加剂变化的稳定性。在无添加剂的情况下储存2周后，这四种分析物相对于初始浓度的活性在4℃时为91.9%至102.8%，25℃时为35.1%至89.6%，37℃时为7.5%至66.9%。抗菌添加剂并不能持续提高稳定性。在 - 80℃无添加剂储存24周的样品，分析物活性范围为69.0%至101.2%。甘油和牛血清白蛋白可提高分析物稳定性；活性范围为91.1%至106.3%。其他添加剂无效。这些结果揭示了在流行病学现场研究期间尿液中生殖激素分析物的储存条件。
