Livesey J H, Roud H K, Metcalf M G, Donald R A
J Endocrinol. 1983 Sep;98(3):381-4. doi: 10.1677/joe.0.0980381.
First morning urine samples were collected from both menstruant and post-menopausal women and stored at -25 degrees C. Immunoreactive FSH disappeared from these samples (t1/2 = 30 days), ultimately stabilizing at about 20% of the initial value. The loss was more rapid at -20 degrees C and less rapid at -55 degrees C and +4 degrees C. Immunoreactive LH was also lost from frozen urine, but more slowly than FSH. The addition of glycerol to urine (0.52 mol/l) stored at -25 degrees C prevented loss of immunoreactive FSH and LH for at least 105 days.
从处于月经期和绝经后的女性中收集首次晨尿样本，并储存在-25摄氏度。这些样本中的免疫反应性促卵泡激素消失（半衰期=30天），最终稳定在初始值的约20%。在-20摄氏度时损失更快，在-55摄氏度和+4摄氏度时损失较慢。冷冻尿液中的免疫反应性促黄体生成素也会损失，但比促卵泡激素慢。向储存在-25摄氏度的尿液中添加甘油（0.52摩尔/升）可防止免疫反应性促卵泡激素和促黄体生成素损失至少105天。
