Török K, Németh K, Erdö F, Arányi P, Székely J I
Institute for Drug Research Ltd., Budapest, Hungary.
Inflamm Res. 1995 Jun;44(6):248-52. doi: 10.1007/BF01782977.
The time-course and pharmacological modulation of interleukin-1 (IL-1) production were investigated during zymosan induced peritonitis in mice. IL-1 alpha liberation was assessed by specific immunoassay (ELISA) and the IL-1 like bioactivity (sensitive to both alpha- and beta-forms of IL-1) was measured by a sensitive bioassay (D10G4.1 costimulation). I.p. injection of zymosan induced significant IL-1 release into the peritoneal exudate. The level peaked at 4 h and by 24 h dropped below the detection limit in both assays. The effects of the prototypical antiinflammatory drugs indomethacin (IND) and dexamethasone (DEX) and that of IX 207-887, a compound which has been reported to interfere primarily with IL-1 production, were also tested. DEX and IX 207-887 dose-dependently decreased the immunoassayable IL-1 alpha level and the IL-1 like bioactivity as well. However, IND had no suppressant effect. Thus, the data obtained by immunoassay and bioassay correlated well proving the suitability of zymosan peritonitis model for the examination of IL-1 production in experimental inflammation.
在酵母聚糖诱导的小鼠腹膜炎期间，研究了白细胞介素-1（IL-1）产生的时间进程和药理调节作用。通过特异性免疫测定法（ELISA）评估IL-1α的释放，并通过灵敏的生物测定法（D10G4.1共刺激）测量IL-1样生物活性（对IL-1的α和β形式均敏感）。腹腔注射酵母聚糖可诱导大量IL-1释放到腹腔渗出液中。该水平在4小时达到峰值，到24小时在两种测定中均降至检测限以下。还测试了典型抗炎药吲哚美辛（IND）和地塞米松（DEX）以及据报道主要干扰IL-1产生的化合物IX 207-887的作用。DEX和IX 207-887剂量依赖性地降低了可免疫测定的IL-1α水平以及IL-1样生物活性。然而，IND没有抑制作用。因此，免疫测定和生物测定获得的数据相关性良好，证明了酵母聚糖腹膜炎模型适用于实验性炎症中IL-1产生的研究。
