Erdö F, Török K, Arányi P, Székely J I
Department of Pharmacology, Institute for Drug Research Ltd., Budapest, Hungary.
Agents Actions. 1993 Jul;39(3-4):137-42. doi: 10.1007/BF01998966.
A new model of local inflammation has been developed: intradermal zymosan-induced mouse ear edema. The symptoms of inflammation induced by injecting zymosan into one of the ears were followed up for 72 h. The ear edema and the local accumulation of polymorphonuclear leukocytes' (PMN) marker enzyme, myeloperoxidase (MPO), were determined. Edema peaked at 4-6 h, while MPO activity peaked at 24 h after zymosan application. The correlation between inflammatory response and concentration of zymosan was also tested. Of the various concentrations tested, 1% suspension has been found optimal. Anti-inflammatory drugs and mediator antagonists were examined in order to establish the selectivity and sensitivity of the assay. A glucocorticoid (dexamethasone), two cyclooxygenase inhibitors (indomethacin, piroxicam) and an interleukin-1 (IL-1) release inhibitor (IX 207-887, Sandoz) all reduced edema and MPO activity as well. However, a lipoxygenase inhibitor (phenidone), a serotonin receptor antagonist (methysergide) and H1 and H2 receptor antagonists (clemastine and cimetidine, respectively) all failed to inhibit the reaction.
皮内注射酵母聚糖诱导的小鼠耳水肿。将酵母聚糖注射到一只耳朵中诱导炎症反应，对其症状进行了72小时的跟踪观察。测定了耳水肿以及多形核白细胞（PMN）标记酶髓过氧化物酶（MPO）的局部蓄积情况。水肿在4 - 6小时达到峰值，而MPO活性在应用酵母聚糖后24小时达到峰值。还测试了炎症反应与酵母聚糖浓度之间的相关性。在所测试的各种浓度中，已发现1%的悬液是最佳的。对抗炎药物和介质拮抗剂进行了检测，以确定该检测方法的选择性和敏感性。一种糖皮质激素（地塞米松）、两种环氧化酶抑制剂（吲哚美辛、吡罗昔康）和一种白细胞介素-1（IL-1）释放抑制剂（IX 207 - 887，山德士公司）也都降低了水肿和MPO活性。然而，一种脂氧合酶抑制剂（非那吡啶）、一种5-羟色胺受体拮抗剂（甲基麦角新碱）以及H1和H2受体拮抗剂（分别为氯马斯汀和西咪替丁）均未能抑制该反应。
