Nomura N, Nagase T, Miyajima N, Sazuka T, Tanaka A, Sato S, Seki N, Kawarabayasi Y, Ishikawa K, Tabata S
Kazusa DNA Research Institute, Chiba, Japan.
DNA Res. 1994;1(5):223-9. doi: 10.1093/dnares/1.5.223.
By applying the protocol previously established, we isolated and sequenced full-length cDNA clones longer than 2 kb from cDNA library of human immature myeloid cell line KG-1, and the coding sequences of 40 new genes were predicted. A computer search of the sequences indicated that 29 genes contained sequences with similarities to reported genes in the GenBank/EMBL databases. Significant transmembrane domains were identified in 9 genes, 5 of which harbored multiple hydrophobic regions. Protein motifs that matched those in the PROSITE motif database were identified in 13 genes. In terms of sequence similarities and protein motifs, 5 genes were related to transcriptional factors. Repetitive sequences were found in the 3'-untranslated region of 8 genes. Northern hybridization demonstrated that the expression of 9 genes was tissue-specific, while the remaining 31 genes were expressed ubiquitously. It was also noted that 17 genes yielded different sizes of bands possibly due to either alternative splicing or alternative initiation. The chromosomal location of these genes has been determined.
通过应用先前建立的方案，我们从人未成熟髓系细胞系KG-1的cDNA文库中分离并测序了长度超过2 kb的全长cDNA克隆，并预测了40个新基因的编码序列。对这些序列进行计算机搜索表明，29个基因的序列与GenBank/EMBL数据库中已报道的基因具有相似性。在9个基因中鉴定出了显著的跨膜结构域，其中5个含有多个疏水区域。在13个基因中鉴定出了与PROSITE基序数据库中的基序相匹配的蛋白质基序。就序列相似性和蛋白质基序而言，5个基因与转录因子相关。在8个基因的3'-非翻译区发现了重复序列。Northern杂交表明，9个基因的表达具有组织特异性，而其余31个基因则普遍表达。还注意到，17个基因产生了不同大小的条带，这可能是由于选择性剪接或选择性起始所致。这些基因的染色体定位已经确定。
