Höhler B, Olry R, Mayer B, Kummer W
Institute for Anatomy and Cell Biology, Philipps University, Marburg, Germany.
Histochem Cell Biol. 1995 Jul;104(1):21-8. doi: 10.1007/BF01464782.
Nitric oxide synthase (NOS) has previously been reported in a small population of postganglionic sympathetic neurons in the guinea pig. The present study of paravertebral ganglia and the inferior mesenteric ganglion aimed to classify these neurons according to their content of neuropeptides (calcitonin gene-related peptide, neuropeptide Y, vasoactive intestinal peptide) and the rate-limiting enzyme of catecholamine synthesis, tyrosine hydroxylase, by means of immunohistochemical and histochemical double-labelling techniques. NOS-containing neurons belonged to the non-catecholaminergic population of postganglionic neurons, and partial co-existence was found with neuropeptide Y and vasoactive intestinal peptide immunoreactivities but not with calcitonin gene-related peptide. However, most of the NOS-containing neurons contained none of the neuropeptides, thus representing a hitherto unrecognized population of postganglionic neurons. The findings show that NOS is localized to small but neurochemically highly specific populations of postganglionic neurons, which most likely reflects an association with target- and function-specific pathways.
此前有报道称，豚鼠的一小部分节后交感神经元中存在一氧化氮合酶（NOS）。本研究针对椎旁神经节和肠系膜下神经节，旨在通过免疫组织化学和组织化学双重标记技术，根据神经肽（降钙素基因相关肽、神经肽Y、血管活性肠肽）的含量以及儿茶酚胺合成的限速酶酪氨酸羟化酶，对这些神经元进行分类。含NOS的神经元属于节后神经元的非儿茶酚胺能群体，发现其与神经肽Y和血管活性肠肽免疫反应性部分共存，但与降钙素基因相关肽不共存。然而，大多数含NOS的神经元不含任何神经肽，因此代表了一类迄今未被识别的节后神经元群体。研究结果表明，NOS定位于节后神经元中数量少但神经化学高度特异的群体，这很可能反映了其与靶标和功能特异性通路的关联。
