Alm P, Uvelius B, Ekström J, Holmqvist B, Larsson B, Andersson K E
Department of Pathology, University of Lund, Lund University Hospital, Sweden.
Histochem J. 1995 Oct;27(10):819-31.
In rats, the distribution of nerve structures staining for NADPH-diaphorase, and showing immunoreactivities for nitric oxide synthase (NOS), tyrosine hydroxylase and various neuropeptides was studied in sensory ganglia (dorsal root, nodose and trigeminal ganglia), in sympathetic ganglia (superior cervical, stellate, coeliac-superior and inferior mesenteric ganglia), parasympathetic ganglia (sphenopalatine, submandibular, sublingual and otic ganglia), and in the mixed parasympathetic/sympathetic ganglia (major pelvic ganglia). The coincidence of neuronal cell bodies with strong NOS-immunoreactivity and strong NADPH diaphorase reactivity was almost total. The relative proportions of NOS-immunoreactive nerve cell bodies were largest in parasympathetic ganglia and major pelvic ganglia followed by sensory ganglia. In sympathetic ganglia no NOS-immunoreactive neuronal cell bodies could be detected. In parasympathetic and major pelvic ganglia, there was a very significant neuronal co-localization of immunoreactivities for NOS and vasoactive intestinal polypeptide (VIP). This was almost total in major pelvic ganglia, in which NOS-/VIP-immunoreactive nerve cell bodies were separate from sympathetic (tyrosine hydroxylase-/neuropeptide Y-immunoreactive), suggesting that NOS-/VIP-immuno-reactive neurons might also be parasympathetic.
在大鼠中，研究了在感觉神经节（背根神经节、结状神经节和三叉神经节）、交感神经节（颈上神经节、星状神经节、腹腔-肠系膜上神经节和肠系膜下神经节）、副交感神经节（蝶腭神经节、下颌下神经节、舌下神经节和耳神经节）以及副交感/交感混合神经节（盆神经节）中，对还原型辅酶Ⅱ-黄递酶（NADPH-d）呈染色反应并对一氧化氮合酶（NOS）、酪氨酸羟化酶和各种神经肽呈免疫反应的神经结构的分布情况。具有强NOS免疫反应性和强NADPH-d反应性的神经元细胞体几乎完全重合。NOS免疫反应性神经细胞体的相对比例在副交感神经节和盆神经节中最大，其次是感觉神经节。在交感神经节中未检测到NOS免疫反应性神经元细胞体。在副交感神经节和盆神经节中，NOS和血管活性肠肽（VIP）的免疫反应性存在非常显著的神经元共定位。在盆神经节中这种共定位几乎是完全的，其中NOS/VIP免疫反应性神经细胞体与交感神经（酪氨酸羟化酶/神经肽Y免疫反应性）的神经细胞体是分开的，这表明NOS/VIP免疫反应性神经元可能也是副交感神经。
