Karawajczyk M, Pauksen K, Peterson C G, Eklund E, Venge P
Department of Clinical Chemistry, University Hospital, Uppsala, Sweden.
Clin Exp Allergy. 1995 Aug;25(8):713-9. doi: 10.1111/j.1365-2222.1995.tb00008.x.
Earlier in vitro studies have suggested that the eosinophil may release its granule proteins selectively depending on the stimulus to which the cell is exposed.
The object of the present study was to study the question of selective release in vivo by means of serum measurements of the two eosinophil granule proteins eosinophil cationic protein (ECP) and eosinophil peroxidase (EPO) in acute infections.
Fourty-six subjects with acute infections were studied before treatment, 20 with bacterial infections and 26 with viral infections. Serum ECP, EPO and MPO were measured by specific RIA.
In acute bacterial infections ECP, but not EPO, was significantly raised in serum (P < 0.0001) compared with non-infected healthy subjects. In acute bacterial infections ECP was significantly correlated to the levels of the neutrophil marker myeloperoxidase (MPO) (rs = 0.96, P < 0.0001) but not to EPO. In acute viral infections neither ECP nor EPO were on average raised. However, almost 20% the patients had elevated levels of bot proteins. In the viral infections the serum-levels of ECP and EPO were correlated (rs = 0.63, P < 0.001), but no correlation was found with MPO.
It is concluded that eosinophils are activated during acute bacterial infections and that this activation results in the preferential mobilisation of ECP. The simultaneous assay of the two eosinophil proteins, ECP and EPO, may give new insight into the role of the eosinophil in disease.
早期的体外研究表明，嗜酸性粒细胞可能会根据细胞所接触的刺激因素选择性地释放其颗粒蛋白。
本研究的目的是通过检测急性感染患者血清中两种嗜酸性粒细胞颗粒蛋白——嗜酸性粒细胞阳离子蛋白（ECP）和嗜酸性粒细胞过氧化物酶（EPO），来研究体内选择性释放的问题。
对46例急性感染患者在治疗前进行研究，其中20例为细菌感染，26例为病毒感染。采用特异性放射免疫分析法检测血清ECP、EPO和MPO。
与未感染的健康受试者相比，急性细菌感染患者血清中ECP显著升高（P < 0.0001），而EPO未升高。在急性细菌感染中，ECP与中性粒细胞标志物髓过氧化物酶（MPO）水平显著相关（rs = 0.96，P < 0.0001），但与EPO无关。在急性病毒感染中，ECP和EPO平均水平均未升高。然而，近20%的患者两种蛋白水平均升高。在病毒感染中，血清ECP和EPO水平相关（rs = 0.63，P < 0.001），但与MPO无相关性。
得出结论，嗜酸性粒细胞在急性细菌感染期间被激活，这种激活导致ECP的优先动员。同时检测两种嗜酸性粒细胞蛋白ECP和EPO，可能会为嗜酸性粒细胞在疾病中的作用提供新的见解。
