Mori M, Inamochi K, Araie M
Department of Ophthalmology, University of Tokyo School of Medicine, Japan.
Curr Eye Res. 1995 Jul;14(7):593-9. doi: 10.3109/02713689508998407.
To find an appropriate way of using Kowa's laser flare-cell meter in evaluating the anterior chamber albumin concentration, we characterized the relationship among the flare intensity determined with the flare-cell meter in terms of photon count/ms (flare value), flare value converted to albumin concentration, and anterior chamber albumin concentration in drug-treated rabbit eyes. Flare measurement was done in rabbits that received intravenous administration of fluorescein isothiocyanate-labeled albumin and instillation of prostaglandin E1, pilocarpine, or tropicamide. Flare value, anterior chamber fluorescence, plasma fluorescence, and plasma albumin concentration were determined. Anterior chamber albumin concentration was calculated from the plasma albumin concentration and the corrected aqueous/plasma fluorescence ratio. The correlation between the flare value converted to albumin concentration (Albequiv) and the anterior chamber albumin concentration (Albac) had two phases: 1) Linear correlation was found when the flare value was below 100 photon count/ms: Albac = 0.53 Albequiv - 0.006, r = 0.98; 2) When the flare value was above 100 photon count/ms, the correlation was lower and had a different slope. In this condition, the Albac was correlated better with the flare value itself: Albac = 0.0042 [flare value] + 0.17, r = 0.88. The results suggested that anterior chamber albumin concentration is evaluated quantitatively from the converted flare value when the flare value is below 100 photon count/ms and from the flare value itself when the flare value is above that level.
为找到一种合适的方法，使用柯瓦（Kowa）激光闪光细胞仪评估前房白蛋白浓度，我们对药物处理的兔眼进行了研究，确定了闪光细胞仪所测定的闪光强度（以光子计数/毫秒表示，即闪光值）、转换为白蛋白浓度的闪光值以及前房白蛋白浓度之间的关系。在静脉注射异硫氰酸荧光素标记白蛋白并滴注前列腺素E1、毛果芸香碱或托吡卡胺的兔子身上进行闪光测量。测定闪光值、前房荧光、血浆荧光和血浆白蛋白浓度。根据血浆白蛋白浓度和校正后的房水/血浆荧光比率计算前房白蛋白浓度。转换为白蛋白浓度的闪光值（Albequiv）与前房白蛋白浓度（Albac）之间的相关性分为两个阶段：1）当闪光值低于100光子计数/毫秒时，呈线性相关：Albac = 0.53 Albequiv - 0.006，r = 0.98；2）当闪光值高于100光子计数/毫秒时，相关性较低且斜率不同。在这种情况下，Albac与闪光值本身的相关性更好：Albac = 0.0042 [闪光值] + 0.17，r = 0.88。结果表明，当闪光值低于100光子计数/毫秒时，可根据转换后的闪光值定量评估前房白蛋白浓度；当闪光值高于该水平时，则根据闪光值本身进行评估。
