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牛头犬蚁(猛蚁属:蚁科)端粒序列的荧光原位杂交分析。

FISH analysis of the telomere sequences of bulldog ants (Myrmecia: formicidae).

作者信息

Meyne J, Hirai H, Imai H T

机构信息

Life Sciences Division and Center for Human Genome Studies, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.

出版信息

Chromosoma. 1995 Oct;104(1):14-8. doi: 10.1007/BF00352221.

DOI:10.1007/BF00352221
PMID:7587589
Abstract

Chromosomes from several species of ants from the genus Myrmecia were hybridized with deoxyoligomer probes of either (T2AG2)7, the putative insect telomere repeat sequence, or (T2AG3)7, the vertebrate telomere repeat sequence. While both sequences hybridized over a range of stringency conditions, (T2AG2)n was clearly the predominant sequence at the termini of the Myrmecia chromosomes. No interstitial sites of either sequence were detected. The genus Myrmecia has a wide range of karyotypes, with chromosome numbers ranging from 2n=2-84. It has been hypothesized that the ancestral karyotype was 2n=4 and karyotype evolution proceeded with an increase in chromosome number. In the absence of detectable interstitial sites of telomere sequence, it is interesting to speculate on the origin of the new telomeres as the chromosome numbers increased.

摘要

将来自斗牛犬蚁属几种蚂蚁的染色体与假定的昆虫端粒重复序列(T2AG2)7或脊椎动物端粒重复序列(T2AG3)7的脱氧寡聚体探针进行杂交。虽然这两种序列在一系列严格条件下都能杂交,但(T2AG2)n显然是斗牛犬蚁属染色体末端的主要序列。未检测到这两种序列的间质位点。斗牛犬蚁属有广泛的核型,染色体数目从2n = 2到84不等。据推测,其祖先核型为2n = 4,核型进化是随着染色体数目的增加而进行的。在未检测到端粒序列的间质位点的情况下,随着染色体数目增加,推测新端粒的起源是很有意思的。

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