Ruíz-Argüelles A, Díaz-Jouanen E, Alarcón-Segovia D
Arthritis Rheum. 1979 Jan;22(1):59-65. doi: 10.1002/art.1780220109.
Phytohemagglutinin-induced cellular cytotoxicity by normal mononuclear cells (MNC) is inhibited by serum of patients with active untreated systemic lupus erythematosus (SLE) but not by that of patients with untreated inactive SLE or rheumatoid arthritis. Three-hour preincubation of normal MNC with SLE sera suffices to inhibit throughout the assay. Preincubation with phytohemagglutinin (PHA) before addition of sera does not prevent the inhibition. DEAE-cellulose chromatography, Sephadex G-200 fractionation and immunoelectrophoretic analysis have shown the nonimmunoglobulin nature of this factor. It is thermostable and not affected by deoxyribonuclease or ribonuclease treatment. The mitogen-induced cellular cytotoxicity-inhibiting factor seems to be independent of other serum factors capable of blocking PHA-induced blastogenic transformation.
正常单核细胞（MNC）在植物血凝素诱导下产生的细胞毒性，会受到未经治疗的活动性系统性红斑狼疮（SLE）患者血清的抑制，但不会受到未经治疗的非活动性SLE患者或类风湿关节炎患者血清的抑制。正常MNC与SLE血清预孵育3小时就足以在整个检测过程中产生抑制作用。在加入血清之前先用植物血凝素（PHA）预孵育，并不能阻止这种抑制作用。二乙氨基乙基纤维素色谱法、葡聚糖凝胶G - 200分级分离法和免疫电泳分析表明，该因子具有非免疫球蛋白的性质。它具有热稳定性，不受脱氧核糖核酸酶或核糖核酸酶处理的影响。有丝分裂原诱导的细胞毒性抑制因子似乎独立于其他能够阻断PHA诱导的母细胞转化的血清因子。
