Wright J K, Hughes P, Gelsthorpe K, Ward A M, Rowell N R
Ann Rheum Dis. 1981 Feb;40(1):11-7. doi: 10.1136/ard.40.1.11.
An investigation of cell-mediated cytotoxicity in 22 patients with systemic lupus erythematosus (SLE), using both whole blood and purified peripheral blood mononuclear cells (PBM) to measure antibody-dependent (ADCC) and phytohaemagglutinin (PHA)-induced lymphocyte cytotoxicity for Chang liver cells, has revealed 2 distinct abnormalities in patients with active disease. PHA-induced cytotoxicity was found to be selectively reduced in whole blood assays only (P less than 0.05), whereas ADCC was impaired in both whole blood (P = 0.02) and PBM (P less than 0.05) assays, when comparison was made with 52 normal controls. The addition of patients' sera to corresponding assays utilizing control PBM confirmed that the impaired PHA-induced cytotoxicity resulted from circulating inhibitory serum factors. Surprisingly little effect, however, was exerted on ADCC assays. These findings suggest that there is a reduction in numbers and/or functional capacity of Fc-receptor cells in active SLE, which may have pathogenetic implications.
对22例系统性红斑狼疮（SLE）患者的细胞介导细胞毒性进行了研究，使用全血和纯化的外周血单个核细胞（PBM）来检测针对Chang肝细胞的抗体依赖性（ADCC）和植物血凝素（PHA）诱导的淋巴细胞细胞毒性，结果显示活动期疾病患者存在2种明显异常。仅在全血检测中发现PHA诱导的细胞毒性选择性降低（P<0.05），而与52名正常对照相比，全血检测（P = 0.02）和PBM检测（P<0.05）中的ADCC均受损。将患者血清添加到使用对照PBM的相应检测中证实，PHA诱导的细胞毒性受损是由循环抑制性血清因子引起的。然而，对ADCC检测的影响出人意料地小。这些发现表明，活动期SLE患者中Fc受体细胞的数量和/或功能能力降低，这可能具有致病意义。
