Ferrero E, Ferrero M E, Pardi R, Zocchi M R
Laboratory of Adoptive Immunotherapy, Scientific Institute San Raffaele, Milan, Italy.
FEBS Lett. 1995 Nov 6;374(3):323-6. doi: 10.1016/0014-5793(95)01110-z.
In this study we have analyzed the role of the platelet-endothelial cell adhesion molecule-1 (PECAM1) in vascular barrier function. PECAM1 is an immunoglobulin gene superfamily member expressed by endothelial cells at the cell boundaries. Macromolecule permeability assays performed on cell monolayers that express native or transfected PECAM1, indicated that the molecule participates in the establishment and maintenance of vascular barrier function in vitro. This hypothesis was confirmed by the finding that in vivo injection of the specific monoclonal antibody directed against the murine vascular PECAM1 led to a detectable leakage of hepatic and renal blood vessels.
在本研究中，我们分析了血小板内皮细胞黏附分子1（PECAM1）在血管屏障功能中的作用。PECAM1是免疫球蛋白基因超家族成员，由内皮细胞在细胞边界处表达。对表达天然或转染PECAM1的细胞单层进行的大分子通透性测定表明，该分子参与体外血管屏障功能的建立和维持。体内注射针对小鼠血管PECAM1的特异性单克隆抗体导致肝和肾血管出现可检测到的渗漏，这一发现证实了该假说。
