Brasseur F, Rimoldi D, Liénard D, Lethé B, Carrel S, Arienti F, Suter L, Vanwijck R, Bourlond A, Humblet Y
Ludwig Institute for Cancer Research, Brussels, Belgium.
Int J Cancer. 1995 Nov 3;63(3):375-80. doi: 10.1002/ijc.2910630313.
Human genes MAGE-1 and MAGE-3 code for antigens that are recognized on melanoma cells by autologous cytolytic T lymphocytes. These antigens may constitute useful targets for specific anti-tumor immunization of cancer patients, since genes MAGE-1 and MAGE-3 are expressed in a number of tumors of different histological types, but are not expressed in normal adult tissues other than testis. This also applies to genes MAGE-2 and MAGE-4, which are closely related to MAGE-1 and MAGE-3. We have analyzed the expression of these 4 MAGE genes in cutaneous melanoma. Sixteen of 100 primary tumors vs. 69 (48%) of 145 metastases from individual patients expressed MAGE-1. Similar differences in the frequency of gene expression between primary and metastatic tumor samples were observed for MAGE-2, MAGE-3, and MAGE-4. MAGE expression in primary tumors was correlated with tumor thickness: there was a significantly increased frequency in the expression of MAGE-1, -2 and -3 in tumors of greater thickness. Benign and dysplastic nevi, as well as in situ melanomas, did not express any of the 4 MAGE genes.
人类基因MAGE - 1和MAGE - 3编码的抗原可被自身溶细胞性T淋巴细胞识别，这些抗原存在于黑色素瘤细胞上。由于MAGE - 1和MAGE - 3基因在多种不同组织学类型的肿瘤中表达，但在除睾丸外的正常成人组织中不表达，所以这些抗原可能构成癌症患者特异性抗肿瘤免疫的有用靶点。这也适用于与MAGE - 1和MAGE - 3密切相关的MAGE - 2和MAGE - 4基因。我们分析了这4种MAGE基因在皮肤黑色素瘤中的表达情况。100例原发性肿瘤中有16例表达MAGE - 1，而来自个体患者的145例转移瘤中有69例（48%）表达MAGE - 1。对于MAGE - 2、MAGE - 3和MAGE - 4，在原发性和转移性肿瘤样本中也观察到了类似的基因表达频率差异。原发性肿瘤中的MAGE表达与肿瘤厚度相关：在厚度较大的肿瘤中，MAGE - 1、 - 2和 - 3的表达频率显著增加。良性和发育异常痣以及原位黑色素瘤均未表达这4种MAGE基因中的任何一种。
