Burge C M, Skinner S L
Department of Physiology, University of Melbourne, Parkville, Victoria, Australia.
J Appl Physiol (1985). 1995 Aug;79(2):623-31. doi: 10.1152/jappl.1995.79.2.623.
An improved protocol was applied to the use of carboxyhemoglobin (HbCO) saturation for the estimation of body hemoglobin (Hb) mass, red blood cell volume, and blood volume and was appraised in normal volunteers. A body size-related dose of CO (50-90 ml), estimated to change HbCO saturation by 6.5%, was introduced into a low-volume (2.8 liters) closed-circuit rebreathing system and allowed to equilibrate over 10 min. Plots of venous HbCO were characterized by a plateau after 8 min, which remained stable for at least 40 min. No loss of CO from the vascular space was evident. Three estimations of Hb mass at weekly intervals in seven subjects produced a coefficient of variation of 0.8% such that, in the absence of physiological influences, changes as little as 1.5% in Hb mass are detectable. Venesection [498 +/- 16 (SE) ml] in seven subjects was associated with a measured decrease in Hb mass after 1 wk equal to the calculated loss. Blood volume was, however, largely restored by plasma expansion. The method is sensitive and precise. It can be used safely and repeatedly in normal volunteers and hospital patients.
一种改进的方案应用于利用碳氧血红蛋白（HbCO）饱和度来估算人体血红蛋白（Hb）质量、红细胞体积和血容量，并在正常志愿者中进行了评估。将与体型相关剂量的一氧化碳（50 - 90毫升）引入低容量（2.8升）闭路再呼吸系统，预计该剂量可使HbCO饱和度变化6.5%，并使其平衡10分钟。静脉血HbCO的曲线在8分钟后呈现平台期，该平台期至少持续稳定40分钟。未发现有一氧化碳从血管间隙中流失。对7名受试者每周进行一次Hb质量的三次估算，变异系数为0.8%，因此在无生理影响的情况下，可检测到Hb质量低至1.5%的变化。7名受试者进行静脉放血（498 ± 16（标准误）毫升）后，1周后测得的Hb质量下降与计算出的损失量相等。然而，血容量在很大程度上通过血浆扩容得以恢复。该方法灵敏且精确。它可在正常志愿者和医院患者中安全且重复使用。
