Rietz B, Belagyi J, Török B, Jacob R
Institute of Physiology II, University of Tübingen, Germany.
Boll Chim Farm. 1995 Feb;134(2):69-76.
The influence of an intake of garlic powder (1% added to a standard chow for an 8 week period) on the susceptibility to ventricular arrhythmias under radical reperfusion was investigated in the isolated rat heart perfused with a modified Krebs-Henseleit solution and the generating system hypoxanthine/xanthine oxidase. The incidence of ventricular fibrillation (VF) after the reopening of the LAD was significantly reduced in the garlic group as compared to the untreated controls (VF: 50% vs 89%). As this protective effect might relate to radical scavenging capacities, two in vitro radical generating test systems were chosen where the garlic activity could be determined. Dose-dependently, garlic was able to capture the radicals. Interestingly, only the garlic extract, was active. The ethanol extract hardly showed any radical scavenging ability. According to this result, we concluded that an intact alliin-alliinase system is important for the activity of garlic. Further investigations were done with different tissues under oxidative stress conditions. The kinetics of each organ, were measured chemiluminometrically. Especially liver and kidney of garlic fed rats showed inhibiting effects. Finally, an attempt was made to relate these radical scavenging and lipidperoxidation inhibiting effects to respective garlic compounds. Two substances, allylmercaptane and diallyldisulfide, were proposed.
研究了在改良的克雷布斯-亨泽莱特溶液灌注的离体大鼠心脏以及次黄嘌呤/黄嘌呤氧化酶生成系统中，摄入大蒜粉（在标准饲料中添加1%，持续8周）对自由基再灌注下心室心律失常易感性的影响。与未处理的对照组相比，大蒜组在重新开放左冠状动脉后室颤（VF）的发生率显著降低（VF：50%对89%）。由于这种保护作用可能与自由基清除能力有关，因此选择了两个体外自由基生成测试系统来测定大蒜的活性。大蒜能够剂量依赖性地捕获自由基。有趣的是，只有大蒜提取物具有活性。乙醇提取物几乎没有显示出任何自由基清除能力。根据这一结果，我们得出结论，完整的蒜氨酸-蒜氨酸酶系统对大蒜的活性很重要。在氧化应激条件下对不同组织进行了进一步研究。通过化学发光法测量每个器官的动力学。喂食大蒜的大鼠的肝脏和肾脏尤其显示出抑制作用。最后，试图将这些自由基清除和脂质过氧化抑制作用与各自的大蒜化合物联系起来。提出了两种物质，烯丙基硫醇和二烯丙基二硫化物。
