血浆和血清中促凝血因子VIII抗原的免疫放射分析及其在血友病中的降低。成人和胎儿血液的初步研究。

Immunoradiometric assay of procoagulant factor-VIII antigen in plasma and serum and its reduction in haemophilia. Preliminary studies on adult and fetal blood.

作者信息

Peake I R, Bloom A L

出版信息

Lancet. 1978 Mar 4;1(8062):473-5. doi: 10.1016/s0140-6736(78)90137-x.

Abstract

An immunoradiometric assay (I.R.M.A.) for antihaemophilic factor (factor VIII) has been developed which is based on a human inhibitor to procoagulant factor VIII (FVIIIC) which arose in a polytransfused haemophiliac. The assay detected and measured the antigen related to FVIIIC (FVIIICAg) in normal plasma and serum but failed to detect any antigen in samples from five patients with severe haemophilia. In a sixth patient with severe haemophilia a non-parallel dose-response curve was obtained. FVIIICAg was also present in plasma and serum from six samples of cord blood and in two samples of fetal serum obtained after termination of pregnancy. The assay may, therefore, be used to measure FVIIICAg in small serum samples and provides a potential practical method for prenatal diagnosis of haemophilia.

摘要

已开发出一种抗血友病因子（因子VIII）免疫放射分析方法（I.R.M.A.），该方法基于一名多次输血的血友病患者体内产生的一种人促凝血因子VIII（FVIIIC）抑制剂。该分析方法可检测和测量正常血浆和血清中与FVIIIC相关的抗原（FVIIICAg），但未能在五名重度血友病患者的样本中检测到任何抗原。在第六名重度血友病患者中，获得了一条非平行的剂量反应曲线。FVIIICAg也存在于六个脐带血样本的血浆和血清中，以及妊娠终止后获得的两个胎儿血清样本中。因此，该分析方法可用于测量少量血清样本中的FVIIICAg，并为血友病的产前诊断提供了一种潜在的实用方法。