Alzugaray R, Gonzalez Hevia M A, Landeras E, Mendoza M C
Departamento de Biología Funcional, Area Microbiología, Universidad de Oviedo, Spain.
New Microbiol. 1995 Apr;18(2):215-22.
Antimicrobial resistance patterns (ARP), virulence profiles and plasmids in clinical isolates of Yersinia enterocolitica serotype O:3 were studied. The ARP was tested using the disk method. All strains were susceptible to amoxicillin/clavulanic acid, cefoxitin, fosfomycin, gentamicin, kanamycin, neomycin, tetracycline, nalidixic acid, norfloxacin, ciprofloxacin and trimethoprim. All of them presented resistance to ampicillin, all with the exception of one to cephalotin, differing in resistance or susceptibility to chloramphenicol, streptomycin (Sm), sulfadiazine (Sd) and cotrimoxazole. Due to these differences they were grouped into 8 ARP. Twenty-nine strains carried plasmids and were grouped into 5 plasmid profiles. All strains carrying 42 MDa plasmids were positive for virulence tests (calcium dependence, crystal violet binding, Congo red binding and autoagglutination). No correlation between ARP and plasmid profile was found, although small plasmids of 6.5 and 4.1 MDa mediated resistance to Sm-Sd, as was shown by transformation to Escherichia coli strains. DNAs from plasmids were analyzed by restriction enzymes. All 42 MDa plasmids showed identical EcoRI and HindIII profiles. The two 6.5 MDa plasmids showed identical BglII, AvaI and SalI restriction profiles and the four 4.1 Mda plasmids also yielded restriction profiles similar to each other, but different from the 6.5 ones.
对小肠结肠炎耶尔森氏菌O:3血清型临床分离株的抗菌药物耐药模式（ARP）、毒力谱和质粒进行了研究。采用纸片法检测ARP。所有菌株对阿莫西林/克拉维酸、头孢西丁、磷霉素、庆大霉素、卡那霉素、新霉素、四环素、萘啶酸、诺氟沙星、环丙沙星和甲氧苄啶敏感。所有菌株对氨苄西林耐药，除一株外对头孢噻吩均耐药，对氯霉素、链霉素（Sm）、磺胺嘧啶（Sd）和复方新诺明的耐药性或敏感性存在差异。由于这些差异，它们被分为8种ARP。29株携带质粒，被分为5种质粒谱。所有携带42 MDa质粒的菌株毒力试验（钙依赖性、结晶紫结合、刚果红结合和自凝）均为阳性。尽管6.5 MDa和4.1 MDa的小质粒介导对Sm-Sd的耐药性（通过转化到大肠杆菌菌株显示），但未发现ARP与质粒谱之间存在相关性。用限制性内切酶分析质粒DNA。所有42 MDa质粒显示相同的EcoRI和HindIII图谱。两个6.5 MDa质粒显示相同的BglII、AvaI和SalI限制性图谱，四个4.1 Mda质粒也产生彼此相似但与6.5 MDa质粒不同的限制性图谱。
