Carr M A, Brewer A W, Osburn B I
Department of Veterinary Pathology, Immunology and Microbiology, University of California, Davis, USA.
Arch Virol. 1995;140(5):915-25. doi: 10.1007/BF01314967.
Some strains of bluetongue virus cause congenital brain damage in bovine and ovine fetuses, as well as in neonatal mice. Two strains of bluetongue virus serotype 11 (UC-2 and UC-8) which differ in neuroinvasiveness were used to determine the biological basis for this difference. UC-2 and UC-8 were inoculated subcutaneously into newborn mice and virus was titrated from blood, plasma and brain tissues over 14 days. For the invasive UC-8 strain, 50-175 plaque forming units of virus per ml was found associated with the blood cells and no virus was detected in the plasma. The virus was detected in the brain at day one post inoculation, and again at day 7, increasing to day 11. The results indicate that UC-8 was able to reach the brain soon after inoculation and to replicate and/or remain in the blood circulation better than UC-2. Immunohistochemical examination of frozen brain sections revealed a sudden, multifocal appearance of UC-8 at day 9, with more viral antigen seen at days 11 and 13, which was barely detected by day 15. Viral antigen was not associated with blood vessels in the brain, indicating that the viral invasion was not from infected vascular endothelium. No virus was detected in the mice infected with strain UC-2.
某些蓝舌病毒株可导致牛和羊胎儿以及新生小鼠出现先天性脑损伤。使用两种神经侵袭性不同的蓝舌病毒血清型11毒株（UC - 2和UC - 8）来确定这种差异的生物学基础。将UC - 2和UC - 8皮下接种到新生小鼠体内，并在14天内从血液、血浆和脑组织中滴定病毒。对于具有侵袭性的UC - 8毒株，每毫升血液细胞中发现50 - 175个空斑形成单位的病毒，血浆中未检测到病毒。接种后第1天在脑中检测到病毒，第7天再次检测到，到第11天病毒量增加。结果表明，UC - 8接种后能很快到达脑部，并且比UC - 2能更好地在血液循环中复制和/或留存。对冷冻脑切片进行免疫组织化学检查发现，第9天UC - 8突然出现多灶性，第11天和第13天可见更多病毒抗原，到第15天几乎检测不到。病毒抗原与脑中血管无关，表明病毒侵袭并非来自受感染的血管内皮。感染UC - 2毒株的小鼠未检测到病毒。
