Waldvogel A S, Anderson C A, Higgins R J, Osburn B I
Department of Pathology, School of Veterinary Medicine, University of California, Davis.
Vet Pathol. 1987 Sep;24(5):404-10. doi: 10.1177/030098588702400507.
In vivo and in vitro experiments were done to investigate whether the difference in neurovirulence between the two strains of bluetongue virus 11, UC-2 and UC-8, is based on a different capability to gain access to the brain from the subcutaneous inoculation site or on a different tropism for neural cells. In newborn Balb/c mice subcutaneous inoculation of UC-8 at doses between 10(-0.2) plaque forming units (PFU) and 10(4.8) PFU caused a severe necrotizing encephalitis whereas UC-2 at doses of up to 10(4.4) PFU did not affect newborn Balb/c mice. However, intracranial inoculation of 10(2.4) PFU of either virus strain produced severe necrotizing encephalitis. In vitro both virus strains infected dissociated brain cell cultures similarly. Double labelling immunofluorescent staining with markers specific for neural cells did not reveal differences in the target cells for the two viruses. The difference in neurovirulence between UC-2 and UC-8, therefore, appears to be determined by the ability of UC-8 to infect the brain from a subcutaneous inoculation site.
进行了体内和体外实验，以研究蓝舌病毒11的两个毒株UC-2和UC-8之间神经毒力的差异，是基于从皮下接种部位进入大脑的能力不同，还是基于对神经细胞的嗜性不同。在新生Balb/c小鼠中，皮下接种剂量在10^(-0.2) 蚀斑形成单位（PFU）至10^(4.8) PFU之间的UC-8会引起严重的坏死性脑炎，而接种剂量高达10^(4.4) PFU的UC-2对新生Balb/c小鼠没有影响。然而，颅内接种10^(2.4) PFU的任一病毒株都会产生严重的坏死性脑炎。在体外，两种病毒株对解离的脑细胞培养物的感染情况相似。用神经细胞特异性标记物进行的双重标记免疫荧光染色未显示两种病毒的靶细胞有差异。因此，UC-2和UC-8之间神经毒力的差异似乎取决于UC-8从皮下接种部位感染大脑的能力。
