用血小板活化因子（PAF）处理的精子体外受精的兔卵母细胞胚胎发育增强。

Enhanced embryo development of rabbit oocytes fertilized in vitro with platelet activating factor (PAF)-treated spermatozoa.

作者信息

Roudebush W E, Fukuda A I, Minhas B S

机构信息

Department of Obstetrics and Gynecology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City.

出版信息

J Assist Reprod Genet. 1993 Jan;10(1):91-4. doi: 10.1007/BF01204447.

DOI:10.1007/BF01204447

PMID:8499686

Abstract

PURPOSE

The purpose of this study was to determine the effects of PAF treatment of rabbit spermatozoa on in vitro fertilization and subsequent blastocyst formation. Rabbit spermatozoa were exposed to PAF (10(-7) M), lyso-PAF (10(-7) M), or HIS (385 mOsm/kg) for 15 min prior to insemination of ovulated oocytes. Fertilized oocytes were cultured to the hatched blastocyst stage.

RESULTS

Fertilization rates with PAF were significantly higher than those of fresh (P < 0.001), lyso-PAF-treated (P < 0.01), HIS-treated (P < 0.05) spermatozoa. Two-cell embryos produced from oocytes inseminated with PAF-treated spermatozoa had significantly higher hatched blastocysts than oocytes inseminated with fresh (P < 0.01), lyso-PAF-treated (P < 0.05), or HIS-treated (P < 0.05) spermatozoa.

CONCLUSION

We conclude that PAF treatment of spermatozoa increases fertilization rates and subsequent embryonic development.

摘要

目的

本研究的目的是确定血小板活化因子（PAF）处理兔精子对体外受精及随后囊胚形成的影响。在给排卵的卵母细胞授精前，将兔精子暴露于PAF（10⁻⁷M）、溶血PAF（10⁻⁷M）或组氨酸（HIS，385毫渗量/千克）中15分钟。将受精的卵母细胞培养至孵化囊胚阶段。

结果

用PAF处理的精子的受精率显著高于新鲜精子（P<0.001）、溶血PAF处理的精子（P<0.01）、HIS处理的精子（P<0.05）。用PAF处理的精子授精的卵母细胞产生的二细胞胚胎，其孵化囊胚显著多于用新鲜精子（P<0.01）、溶血PAF处理的精子（P<0.05）或HIS处理的精子（P<0.05）授精的卵母细胞。