放射性标记的玉米赤霉烯酮与禾谷镰刀菌蛋白质组分的优先结合。
Preferential binding of radiolabeled zearalenone to a protein fraction of Fusarium roseum graminearum.
作者信息
Inaba T, Mirocha C J
出版信息
Appl Environ Microbiol. 1979 Jan;37(1):80-4. doi: 10.1128/aem.37.1.80-84.1979.
Zearalenone [6-(10-hydroxy-6-oxo-trans-1-undecenyl)beta-resorcyclic acid lactone] is a hormone produced by Fusarium spp. which regulates the sexual stage in F. roseum. 3H- and 14C-labeled zearalenone were found to bind preferentially to one of two peaks containing uncharacterized proteins obtained from the cytosol of young mycelium and resolved by gel column chromatography. The proteins were partially purified by successive resolution on Sephadex G-100, Sephadex G-200, and BioGel P-300. Free zearalenone (37%) was reisolated from the purified proteins after resolution by thin-layer chromatography or partitioning with ethyl acetate.
玉米赤霉烯酮[6-(10-羟基-6-氧代-反式-1-十一碳烯基)β-间苯二酚环酸内酯]是一种由镰刀菌属产生的激素，它调节粉红镰刀菌的有性阶段。研究发现，用³H和¹⁴C标记的玉米赤霉烯酮优先结合从幼龄菌丝体细胞质中获得的、经凝胶柱色谱分离得到的两个含有未鉴定蛋白质的峰中的一个。通过在Sephadex G-100、Sephadex G-200和BioGel P-300上连续分离，对这些蛋白质进行了部分纯化。通过薄层色谱或用乙酸乙酯分配进行分离后，从纯化的蛋白质中重新分离出游离的玉米赤霉烯酮(37%)。
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