Kallela K, Saastamoinen I
Nord Vet Med. 1981 Sep-Nov;33(9-11):454-60.
The studies illustrate the effects of preservatives - "Luprosil" (propionic acid) and "Gasol" (contains organic acids and some additional compounds) on the growth of Fusarium graminearum and the quality of zearalenone in contaminated grain. The laboratory conditions resembled, concerning the supply of oxygen, the circumstances in the surface layers of preserved grain mass. The mycelium growth of the fungus Fusarium graminearum was visually observed. Three successions of studies of grain cultures were carried out before addition of preservatives and after 3, 14 and 28 days of storage: 1) liquid-chromatographic examination indicating the amount of soluble zearalenone in oat-wheat culture 2) bioassay indicating the amount of insoluble zearalenone in grain (solid residue) 3) bioassay indicating the total amount of zearalenone (insoluble + soluble zearalenone) in oat-wheat-barley culture. The preservatives completely prevented the visible mycelium growth of Fusarium graminearum. Propionic acid ("Luprosil") had no reducing effect on the quantity of zearalenone. "Gasol", on the contrary, reduced the amount of the mycotoxin very clearly. The decrease in different successions of studies was after 3, 14 and 28 days from the commencement of storage as follows: 1) 60, 90 and 95% 2) 15, 17 and 90% 3) 70, 80 and 90%.
这些研究阐述了防腐剂“Luprosil”（丙酸）和“Gasol”（含有有机酸及一些其他化合物）对禾谷镰刀菌生长以及受污染谷物中玉米赤霉烯酮质量的影响。在氧气供应方面，实验室条件类似于保存谷物堆表层的情况。肉眼观察了禾谷镰刀菌的菌丝体生长。在添加防腐剂之前以及储存3天、14天和28天后，对谷物培养物进行了三轮研究：1）液相色谱检测，测定燕麦 - 小麦培养物中可溶性玉米赤霉烯酮的含量；2）生物测定，测定谷物中不溶性玉米赤霉烯酮的含量（固体残渣）；3）生物测定，测定燕麦 - 小麦 - 大麦培养物中玉米赤霉烯酮的总量（不溶性 + 可溶性玉米赤霉烯酮）。防腐剂完全抑制了禾谷镰刀菌肉眼可见的菌丝体生长。丙酸（“Luprosil”）对玉米赤霉烯酮的含量没有降低作用。相反，“Gasol”能显著降低霉菌毒素的含量。在储存开始后的3天、14天和28天，不同轮次研究中的降低幅度如下：1）60%、90%和95%；2）15%、17%和90%；3）70%、80%和90%。
