Doehring E, Reiter-Owona I, Bauer O, Kaisi M, Hlobil H, Quade G, Hamudu N A, Seitz H M
Institute of Medical Parasitology, University of Bonn, Germany.
Am J Trop Med Hyg. 1995 Jun;52(6):546-8. doi: 10.4269/ajtmh.1995.52.546.
The sera of 849 Tanzanian pregnant women were tested at delivery for Toxoplasma gondii antibodies with the Sabin-Feldman dye test (DT) and an immunosorbent agglutination assay. A total of 296 (35%) of these women had DT titers greater than 1:4. The percentage of women with dye test titers greater than 1:4 was 34-37% regardless of the individual ages. The rate of positivity for human immunodeficiency virus 1/2 (HIV-1/2) using Western blotting was 11.5%. There was no relationship between prevalence of a positive DT result and HIV infection nor between the intensity of the DT result and HIV infection. Sixty-four parturients had a DT titer of 1:1,000 or more. From 57 newborns of these mothers, cord sera were available and were screened by the DT and the immunosorbent agglutination assay. Seven of these were found to be positive for IgM and/or IgA antibodies. It was concluded that the rate of serologic evidence for prenatal Toxoplasma infection in cord blood samples in the present study of Tanzanian pregnant women was approximately 0.8%.
在分娩时，采用Sabin - Feldman染色试验（DT）和免疫吸附凝集试验对849名坦桑尼亚孕妇的血清进行了弓形虫抗体检测。这些孕妇中共有296名（35%）的DT滴度大于1:4。无论个体年龄如何，染色试验滴度大于1:4的女性比例为34 - 37%。采用蛋白质印迹法检测人类免疫缺陷病毒1/2（HIV - 1/2）的阳性率为11.5%。DT结果阳性率与HIV感染之间以及DT结果强度与HIV感染之间均无关联。64名产妇的DT滴度为1:1000或更高。从这些母亲的57名新生儿中获取了脐带血清，并通过DT和免疫吸附凝集试验进行筛查。其中7名被发现IgM和/或IgA抗体呈阳性。得出的结论是，在本项对坦桑尼亚孕妇的研究中，脐带血样本中产前弓形虫感染的血清学证据发生率约为0.8%。
