从无乳链球菌中分离出一种新的HaeIII同裂酶SagI。
Isolation of SagI, a new HaeIII isoschizomer from Streptococcus agalactiae.
作者信息
Poch M T, Somkuti G A
机构信息
U. S. Department of Agriculture, Agricultural Research Service, Philadelphia, PA 19118, USA.
出版信息
Appl Microbiol Biotechnol. 1995 May-Jun;43(2):282-4. doi: 10.1007/BF00172825.
PMID:7612245
Abstract
A new HaeIII isoschizomer from Streptococcus agalactiae was isolated by a single-step purification method. The highly active restriction endonuclease, SagI, was free of nonspecific nuclease activity and was suitable for use in molecular biology procedures. The rapid isolation procedure may be applicable for the recovery of other restriction endonucleases from bacteria.
摘要
通过单步纯化方法从无乳链球菌中分离出一种新的HaeIII同裂酶。这种高活性的限制性内切酶SagI没有非特异性核酸酶活性，适用于分子生物学操作。这种快速分离程序可能适用于从细菌中回收其他限制性内切酶。
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