Lau C O, Tan C H, Khoo H E, Li Q T, Yuen R
Department of Biochemistry, Faculty of Medicine, National University of Singapore.
Nat Toxins. 1995;3(2):87-90. doi: 10.1002/nt.2620030205.
A purification procedure for Lophozozymus pictor toxin (LPTX) following ethanolic extraction of whole crab homogenate is described. The ethanol-extracted toxin (LPTX-E) had higher yield and specific activity than the hot aqueous-extracted one (LPTX-H). It was found that LPTX-E was fluorescent and cochromatographed with LPTX-H on two-dimensional thin-layer chromatography. Although LPTX-E, LPTX-H, and palytoxin (P. caribaeorum, PTX) had similar migration and retention times when analysed on high performance capillary electrophoresis and gel permeation-high performance liquid chromatography respectively, LPTX-E and LPTX-H were both fluorescent in contrast to PTX. In addition, LPTX-E had a different retention time compared with PTX when chromatographed on reversed phase high performance liquid chromatography in the solvent system 80% acetonitrile and 0.02 M Tris-HCl, pH 7.2, at a 4:1 ratio, respectively, indicating some differences in their chemical structures.
本文描述了一种从全蟹匀浆乙醇提取物中纯化斑纹龙虾毒素（LPTX）的方法。乙醇提取的毒素（LPTX-E）比热水提取的毒素（LPTX-H）具有更高的产量和比活性。研究发现，LPTX-E具有荧光性，并且在二维薄层色谱上与LPTX-H共色谱。尽管分别在高效毛细管电泳和凝胶渗透-高效液相色谱上分析时，LPTX-E、LPTX-H和岩沙海葵毒素（加勒比海岩沙海葵，PTX）具有相似的迁移和保留时间，但与PTX不同，LPTX-E和LPTX-H均具有荧光性。此外，当在80%乙腈和0.02 M Tris-HCl（pH 7.2）的溶剂系统中以4:1的比例在反相高效液相色谱上进行色谱分析时，LPTX-E与PTX的保留时间不同，这表明它们的化学结构存在一些差异。
