一种用于检测局限性黏附性经典肠致病性大肠杆菌血清群的酶联免疫吸附测定法。

An ELISA for the detection of localized adherent classic enteropathogenic Escherichia coli serogroups.

作者信息

Albert M J, Ansaruzzaman M, Faruque S M, Neogi P K, Haider K, Tzipori S

机构信息

International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka.

出版信息

J Infect Dis. 1991 Nov;164(5):986-9. doi: 10.1093/infdis/164.5.986.

DOI:10.1093/infdis/164.5.986

PMID:1940480

Abstract

An ELISA for the detection of classic enteropathogenic Escherichia coli (EPEC) serogroups was developed. It detected EPEC positive for localized adherence (LA) by HeLa cell assay and EPEC positive for EPEC adherence factor (EAF) by DNA probe assay. A specific antiserum was raised with LA+ EPEC strain E2348/69 (serotype O127:H6) by immunizing rabbits and then absorbing the antiserum with its LA- derivative, MAR20. The absorbed antiserum reacted specifically with all 90 strains of E. coli belonging to eight different EPEC serogroups that were LA+ by HeLa cell assay and EAF+ by DNA probe assay. All E. coli strains including EPEC serogroups that were LA- by HeLa cell assay and EAF- by DNA probe assay were also negative by ELISA. Thus the ELISA is 100% sensitive and specific in detecting LA+ classic EPEC serogroups.

摘要

开发了一种用于检测典型肠致病性大肠杆菌（EPEC）血清群的酶联免疫吸附测定（ELISA）。该方法通过HeLa细胞试验检测出局部黏附（LA）呈阳性的EPEC，通过DNA探针检测法检测出EPEC黏附因子（EAF）呈阳性的EPEC。用LA+ EPEC菌株E2348/69（血清型O127:H6）免疫兔子，然后用其LA-衍生物MAR20吸收抗血清，从而制备出特异性抗血清。吸收后的抗血清与通过HeLa细胞试验检测为LA+且通过DNA探针检测为EAF+的属于八个不同EPEC血清群的所有90株大肠杆菌发生特异性反应。所有大肠杆菌菌株，包括通过HeLa细胞试验检测为LA-且通过DNA探针检测为EAF-的EPEC血清群，通过ELISA检测也均为阴性。因此，ELISA在检测LA+典型EPEC血清群方面具有100%的敏感性和特异性。