Morrison L D, Becker L, Ang L C, Kish S J
Human Neurochemical Pathology Laboratory, Clarke Institute of Psychiatry, Toronto, Ontario, Canada.
J Neurochem. 1995 Aug;65(2):636-42. doi: 10.1046/j.1471-4159.1995.65020636.x.
Although much evidence has implicated polyamines in brain development and function, little information is available on these substances in human brain. We examined the influence of regional distribution and aging on putrescine, spermidine, and spermine levels in autopsied human brain. In the adult brain, concentrations of spermidine were the highest, followed by spermine and putrescine. All three polyamines showed a distinct and uneven distribution profile among the 10 examined brain areas. Spermidine levels were especially high in white matter and thalamus (20 and 9.3 nmol/mg of protein, respectively), whereas spermine concentrations were highest in cerebellar cortex (3.4 nmol/mg of protein). High levels of putrescine were observed in cerebral cortices, putamen, and hippocampus (0.7-1.2 nmol/mg or protein), with lowest levels in cerebellum and thalamus (0.3-0.5 nmol/mg of protein). No statistically significant influence of aging (1 day to 103 years; n = 57) on either putrescine or spermine levels in occipital cortex was observed. In contrast, spermidine levels increased markedly from birth, reaching maximal levels at approximately 40 years of age (+228% increase in the mean 41-year-old group vs. 6-week-old group), which were maintained up to senescence. These observations in human brain thus differ from those reported in the rodent, in which levels of all three polyamines show a pronounced postnatal reduction. Our data support the notion that polyamines may have roles in both postnatal brain development and in mature brain function.
尽管有大量证据表明多胺与大脑发育和功能有关，但关于这些物质在人类大脑中的信息却很少。我们研究了区域分布和衰老对尸检人类大脑中腐胺、亚精胺和精胺水平的影响。在成人大脑中，亚精胺的浓度最高，其次是精胺和腐胺。在检查的10个脑区中，所有三种多胺都呈现出明显且不均匀的分布模式。亚精胺水平在白质和丘脑中特别高（分别为20和9.3 nmol/mg蛋白质），而精胺浓度在小脑皮质中最高（3.4 nmol/mg蛋白质）。在大脑皮质、壳核和海马体中观察到高水平的腐胺（0.7 - 1.2 nmol/mg或蛋白质），在小脑和丘脑中水平最低（0.3 - 0.5 nmol/mg蛋白质）。未观察到衰老（1天至103岁；n = 57）对枕叶皮质中腐胺或精胺水平有统计学显著影响。相比之下，亚精胺水平从出生时就显著增加，在大约40岁时达到最高水平（41岁平均组与6周龄组相比增加了228%），并一直维持到衰老。因此，人类大脑中的这些观察结果与啮齿动物中报道的不同，在啮齿动物中，所有三种多胺的水平在出生后都有明显下降。我们的数据支持多胺可能在出生后大脑发育和成熟大脑功能中都起作用的观点。
