Choi M S, Kwak S S, Liu J R, Park Y G, Lee M K, An N H
Bioresources Research Group, Genetic Engineering Research Institute, KIST, Yusong, Taejon, Korea.
Planta Med. 1995 Jun;61(3):264-6. doi: 10.1055/s-2006-958069.
The concentrations of taxol and related compounds in the bark and needles of Taxus cuspidata grown on Mt. Jiri, Mt. Sobaek, and Cheju Island, and T. cuspidata var. latifolia on Ullung Island in Korea were determined by high performance liquid chromatography (HPLC). The taxane content significantly varied with the location and plant part. The taxol content in the bark of native yews from Mt. Jiri and Mt. Sobaek was high when compared to that reported for Pacific yew (T. brevifolia), whereas bark from trees on Cheju and Ullung islands contained a much lower level. Surprisingly, the needles from Cheju and Ullung islands contained a much higher level of taxol (0.022% and 0.0173%, respectively) than those of intermountain locations (0.0058% to 0.0085%), on the basis of dry weight. The bark and needles of T. cuspidata var. latifolia on Ullung Island also contained relatively high concentrations of 10-deacetylbaccatin III, 0.0497% and 0.0545%, respectively, and indicated that environmental factors may affect the quantity. Taxol in the needles was confirmed by electrospray mass spectrometry. These results suggest that foliage from yew trees growing in their natural habitats on Cheju and Ullung islands may provide a renewable source for taxol.
采用高效液相色谱法（HPLC）测定了生长在韩国智异山、小白山和济州岛以及郁陵岛的宽叶紫杉树皮和针叶中紫杉醇及相关化合物的浓度。紫杉烷含量随产地和植物部位的不同而有显著差异。与报道的太平洋紫杉（短叶红豆杉）相比，来自智异山和小白山的原生紫杉树皮中的紫杉醇含量较高，而济州岛和郁陵岛树木的树皮中紫杉醇含量则低得多。令人惊讶的是，以干重计，济州岛和郁陵岛的针叶中紫杉醇含量（分别为0.022%和0.0173%）比山间地区的针叶（0.0058%至0.0085%）高得多。郁陵岛宽叶紫杉的树皮和针叶中10 - 去乙酰巴卡亭III的含量也相对较高，分别为0.0497%和0.0545%，这表明环境因素可能会影响其含量。通过电喷雾质谱法确认了针叶中的紫杉醇。这些结果表明，生长在济州岛和郁陵岛自然栖息地的紫杉树叶可能为紫杉醇提供可再生来源。
