Doyle I R, Marshall V R, Dawson C J, Ryall R L
Department of Surgery, Flinders Medical Centre, Bedford Park, South Australia.
Urol Res. 1995;23(1):53-62. doi: 10.1007/BF00298852.
Demineralization of calcium oxalate (CaOx) crystals precipitated from human urine in vitro yields an organic crystal matrix extract (CME) consisting predominantly of a single protein which we originally named crystal matrix protein but have subsequently shown to be a urinary form of prothrombin activation peptide fragment 1 (F1). The aim of this study was to determine whether CME is a promoter or inhibitor of CaOx crystallization. The effect of CME on CaOx crystal growth and aggregation was tested using a standard seeded crystallization system, and its effect quantified by use of particle size analysis and a computer model. In addition, the effect of CME on the crystallization of CaOx was tested in undiluted, ultrafiltered human urine using Coulter Counter analysis and scanning electron microscopy. It was shown that CME is a potent inhibitor of CaOx crystal growth and aggregation in a seeded metastable solution. However, of greater significance is that at a concentration of 10 mg/l it completely reversed the formation of large crystalline aggregates that form upon the removal of urinary macromolecules from undiluted urine. It was concluded that CME is the most potent macromolecular urinary inhibitor yet to be tested in urine in vitro. By preventing the aggregation of newly formed crystals, the components of CME may significantly reduce the probability of particle retention in vivo and therefore the occurrence of urolithiasis.
从人尿液中体外沉淀的草酸钙（CaOx）晶体脱矿质后可产生一种有机晶体基质提取物（CME），其主要由一种单一蛋白质组成，我们最初将其命名为晶体基质蛋白，但随后发现它是凝血酶原激活肽片段1（F1）的一种尿液形式。本研究的目的是确定CME是促进还是抑制CaOx结晶。使用标准的晶种结晶系统测试CME对CaOx晶体生长和聚集的影响，并通过粒度分析和计算机模型对其效果进行量化。此外，使用库尔特计数器分析和扫描电子显微镜在未稀释的超滤人尿液中测试CME对CaOx结晶的影响。结果表明，CME在亚稳溶液晶种中是CaOx晶体生长和聚集的有效抑制剂。然而，更重要的是，在浓度为10 mg/l时，它完全逆转了从未稀释尿液中去除尿大分子后形成的大结晶聚集体的形成。得出的结论是，CME是体外尿液中测试过的最有效的大分子尿抑制剂。通过防止新形成的晶体聚集，CME的成分可能会显著降低体内颗粒滞留的可能性，从而降低尿路结石的发生率。
