Lutz H, Castelli I, Ehrensperger F, Pospischil A, Rosskopf M, Siegl G, Grob M, Martinod S
Department of Veterinary Medicine, University of Zurich, Switzerland.
Vet Immunol Immunopathol. 1995 May;46(1-2):21-33. doi: 10.1016/0165-2427(94)07003-p.
To study the effect of interferon on feline leukemia virus (FeLV) infection, 30 specific pathogen free (SPF) cats were infected with the apathogenic FeLV A Glasgow. Unexpectedly, between 5 and 8 weeks after FeLV infection, all 19 cats with persistent FeLV infection but not the FeLV-negative cats died from a panleukopenia-like syndrome. No feline panleukopenia virus (FPLV) antigen was found in feces by latex agglutination, enzyme-linked immunosorbent assay (ELISA) or immunoelectron microscopy. No enteropathogenic bacteria were found. Histopathology revealed changes resembling those of FPLV infection such as destruction of crypts and pancytopenia of bone marrow. Neither clinical signs nor seroconversion to FPLV could be induced by transmitting intestinal extracts to two SPF cats. However, FPLV antigen was demonstrated by immunofluorescence assay in intestinal cryostat sections of diseased animals. FPLV could also be demonstrated in intestinal extracts by immunoelectron microscopy, by latex agglutination and ELISA after anti-FPLV antibodies were removed from immune-complexed FPLV by ultracentrifugation over a CsCl gradient at pH 2.0. From these experiments it was concluded that the panleukopenia-like syndrome of FeLV may not be caused by FeLV alone but at least in some cases by co-infection with FeLV and FPLV. In addition, some form of 'cooperation' between FeLV and FPLV must be postulated because neither virus alone induced symptoms.
为研究干扰素对猫白血病病毒（FeLV）感染的影响，30只无特定病原体（SPF）猫感染了无致病性的FeLV A格拉斯哥株。出乎意料的是，在FeLV感染后5至8周期间，所有19只持续感染FeLV的猫而非FeLV阴性的猫死于一种类似猫泛白细胞减少症的综合征。通过乳胶凝集试验、酶联免疫吸附测定（ELISA）或免疫电子显微镜检查，在粪便中未发现猫泛白细胞减少症病毒（FPLV）抗原。未发现肠道致病细菌。组织病理学显示出类似于FPLV感染的变化，如隐窝破坏和骨髓全血细胞减少。将患病动物的肠道提取物接种到两只SPF猫身上，既未诱发临床症状，也未出现FPLV血清转化。然而，通过免疫荧光测定在患病动物的肠道低温切片中证实了FPLV抗原的存在。通过免疫电子显微镜检查，在肠道提取物中也证实了FPLV的存在，并且在pH 2.0条件下通过氯化铯梯度超速离心从免疫复合物FPLV中去除抗FPLV抗体后，通过乳胶凝集试验和ELISA也证实了FPLV的存在。从这些实验得出结论，FeLV的类似猫泛白细胞减少症的综合征可能并非仅由FeLV引起，而是至少在某些情况下由FeLV和FPLV共同感染所致。此外，必须假定FeLV和FPLV之间存在某种形式的“协同作用”，因为单独任何一种病毒都不会诱发症状。
