Chu K U, Evers B M, Ishizuka J, Townsend C M, Thompson J C
Department of Surgery, University of Texas Medical Branch, Galveston, USA.
Ann Surg. 1995 Jul;222(1):94-100. doi: 10.1097/00000658-199507000-00015.
The authors examined the effects of exogenous bombesin (BBS) on gut mucosal growth in chow-fed rats and the mucosal regeneration after gut atrophy brought about by feeding an elemental diet and after intestinal injury produced by methotrexate (MTX).
Bombesin is one of many gastrointestinal peptides implicated in the regulation of gut mucosal growth. Although BBS is known to stimulate growth of normal pancreatic tissue, the trophic effect of BBS on gut mucosa is less clear and its exact role in gut mucosal regeneration and repair is not known.
Rats were fed a regular chow diet (control) or an elemental diet plus either saline or BBS (10 micrograms/kg). In another experiment, rats fed a chow diet and treated with saline or BBS were given MTX (20 micrograms/kg) or a single intraperitoneal injection. In all experiments, small and large bowel mucosa and pancreas were removed and analyzed for BBS-mediated proliferation.
Bombesin produced significant mucosal proliferation of the small bowel at day 14, but not at day 7, in rats fed regular chow. In contrast, BBS treatment for 7 days produced significant proliferation in both the atrophic and injured gut mucosa of rats given elemental diet or MTX.
Bombesin may be an important enterotrophic factor for normal mucosal proliferation and may be clinically beneficial as an agent to restore or maintain gut mucosa during periods of atrophy or injury.
作者研究了外源性蛙皮素（BBS）对正常进食大鼠肠道黏膜生长的影响，以及在给予要素饮食导致肠道萎缩后和甲氨蝶呤（MTX）造成肠道损伤后肠道黏膜的再生情况。
蛙皮素是众多参与调节肠道黏膜生长的胃肠肽之一。尽管已知BBS可刺激正常胰腺组织生长，但其对肠道黏膜的营养作用尚不清楚，其在肠道黏膜再生和修复中的确切作用也未知。
大鼠分别给予常规普通饲料（对照组）或要素饮食加生理盐水或BBS（10微克/千克）。在另一项实验中，给予普通饲料并接受生理盐水或BBS处理的大鼠注射MTX（20微克/千克）或单次腹腔注射。在所有实验中，切除小肠和大肠黏膜以及胰腺，分析BBS介导的增殖情况。
在给予常规普通饲料的大鼠中，BBS在第14天可使小肠黏膜显著增殖，但在第7天无此作用。相比之下，给予要素饮食或MTX的大鼠，BBS处理7天可使萎缩和损伤的肠道黏膜均显著增殖。
蛙皮素可能是正常黏膜增殖的重要肠营养因子，在萎缩或损伤期间作为恢复或维持肠道黏膜的药物可能具有临床益处。
