Johnson R C, Ely B
J Bacteriol. 1979 Jan;137(1):627-34. doi: 10.1128/jb.137.1.627-634.1979.
A total of 69 spontaneous nonmotile mutants were isolated from the dimorphic bacterium Caulobacter crescentus. The majority of the mutants were unable to assemble a flagellar filament (Fla-), although eight were able to synthesize a short stub of a flagellum. A third mutant class assembled flagella of normal morphology but were nonmotile (Mot-). Genetic analysis by phiCr30-mediated transduction revealed 27 linkage groups for the fla and stub-forming mutations, and three linkage groups for the mot mutations. Intracellular flagellin detected by immunodiffusion was at the limit of detectability in most of the Fla- and stub-forming mutants but normal in the Mot- mutants. The Fla- and stub-forming mutants also showed decreased sensitivity to the swarmer-specific phages phiCbK and phiCb5 and phiCr40. One additional strain was totally resistant to phiCbK, and the mutation in this strain has been designated pleA. Each of the mutants containing mot mutations showed wild-type sensitivity to all of the phages tested.
从双态细菌新月柄杆菌中总共分离出69个自发的无运动能力突变体。大多数突变体无法组装鞭毛丝（Fla-），不过有八个能够合成短的鞭毛残端。第三类突变体组装出形态正常的鞭毛但无运动能力（Mot-）。通过phiCr30介导的转导进行的遗传分析表明，fla和形成残端的突变有27个连锁群，mot突变有三个连锁群。通过免疫扩散检测到的细胞内鞭毛蛋白在大多数Fla-和形成残端的突变体中处于可检测极限，但在Mot-突变体中正常。Fla-和形成残端的突变体对群体特异性噬菌体phiCbK、phiCb5和phiCr40的敏感性也降低。另外有一个菌株对phiCbK完全抗性，该菌株中的突变已被命名为pleA。每个含有mot突变的突变体对所有测试噬菌体都表现出野生型敏感性。
