Tn10的限制性内切酶切割图谱，Tn10是一种编码四环素抗性的转座子。
Restriction enzyme cleavage map of Tn10, a transposon which encodes tetracycline resistance.
作者信息
Jorgensen R A, Berg D E, Allet B, Reznikoff W S
出版信息
J Bacteriol. 1979 Jan;137(1):681-5. doi: 10.1128/jb.137.1.681-685.1979.
Abstract
A cleavage map of a recombinant plasmid carrying Tn10 was constructed for 13 different restriction enzymes. The Tn10 region of this plasmid contains cleavage sites for BamHI, AvaI, BglI, BglII, EcoRI, XbaI, HincII, HindIII, and HpaI. Restriction enzymes PstI, SmaI, KpnI, XhoI, SalI, and PvuI do not cleave within the Tn10 element. This map confirms the previously reported structure of this transposon; it is composed of a unique sequence (approximately6,400 base pairs long), which in part codes for the tetracycline resistance functions and is bounded by inverted repeats (approximately 1,450 base pairs long).
摘要
构建了携带Tn10的重组质粒针对13种不同限制酶的切割图谱。该质粒的Tn10区域包含BamHI、AvaI、BglI、BglII、EcoRI、XbaI、HincII、HindIII和HpaI的切割位点。限制酶PstI、SmaI、KpnI、XhoI、SalI和PvuI不在Tn10元件内切割。该图谱证实了此前报道的该转座子的结构；它由一个独特序列（约6400个碱基对长）组成，该序列部分编码四环素抗性功能，并由反向重复序列（约1450个碱基对长）界定。
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