Smith D I, Blattner F R, Davies J
Nucleic Acids Res. 1976 Feb;3(2):343-53. doi: 10.1093/nar/3.2.343.
We describe the isolation of a new class 2 restriction endonuclease from Providencia stuartii 164. Using the procedure of osmotic shock treatment, we have partially purified this enzyme (Pst 1) and have begun preliminary work to characterize its specificity and requirements. Pst 1 requires Mg++ as the only cofactor and produces more than 18 cleavages in wild type lambda. We have determined the location of 7 of these cleavages by the use of deletion and insertion mutants of lambda.
我们描述了从斯氏普罗威登斯菌164中分离出一种新的II类限制性内切酶的过程。利用渗透休克处理程序，我们已对这种酶（Pst 1）进行了部分纯化，并已开始对其特异性和需求进行初步研究。Pst 1仅需要Mg++作为辅助因子，并且在野生型λ噬菌体中可产生超过18个切割位点。我们通过使用λ噬菌体的缺失和插入突变体确定了其中7个切割位点的位置。
