Held T K, Adamczik C, Trautmann M, Cross A S
Abteilung für Hämatologie und Onkologie, Universitätsklinikum Rudolf Virchow/Charlottenburg, Freie Universität, Berlin, Germany.
Antimicrob Agents Chemother. 1995 May;39(5):1093-6. doi: 10.1128/AAC.39.5.1093.
In the present study, we examined whether MICs and sub-MICs of antimicrobial agents belonging to two different classes, ciprofloxacin and ceftazidime, were able to influence the production and release of cell-associated and soluble (extracellular) capsular polysaccharide (CPS), respectively, in a heavily encapsulated strain of Klebsiella pneumoniae (B5055). Using a CPS-specific enzyme-linked immunosorbent assay, we found that the amount of cell-associated CPS increased in a dose-dependent manner by more than 10-fold under the influence of the MIC of ceftazidime and by more than 100-fold under the influence of the MIC of ciprofloxacin. The largest amounts of CPS were measured by using the MIC of either antibiotic substance. Electron microscopic studies showed that the diameter of the capsule was significantly increased compared with the diameter for untreated controls. Thus, both antimicrobial agents genuinely stimulated CPS production.
在本研究中，我们检测了属于两种不同类别的抗菌药物环丙沙星和头孢他啶的最低抑菌浓度（MIC）及亚MIC是否能够分别影响肺炎克雷伯菌强荚膜菌株（B5055）中与细胞相关的和可溶性（细胞外）荚膜多糖（CPS）的产生与释放。通过使用CPS特异性酶联免疫吸附测定法，我们发现，在头孢他啶MIC的影响下，与细胞相关的CPS量呈剂量依赖性增加，超过10倍；在环丙沙星MIC的影响下，增加超过100倍。使用任一抗生素物质的MIC测得的CPS量最大。电子显微镜研究表明，与未处理对照的直径相比，荚膜直径显著增加。因此，这两种抗菌药物均真正刺激了CPS的产生。
