Williams P
Antimicrob Agents Chemother. 1987 May;31(5):758-62. doi: 10.1128/AAC.31.5.758.
Growth of encapsulated (K+) and nonencapsulated (K-) Klebsiella pneumoniae strains in media containing sub-MICs of either cefuroxime or ciprofloxacin resulted in cell elongation but had little effect on the outer membrane protein or lipopolysaccharide profiles. Exposure to serum complement increased the surface hydrophobicity of a K- strain but failed to interact or to increase the surface hydrophobicity of the K+ strains. However, after growth of the K+ strains in sub-MICs of the antibiotics, complement increased their surface hydrophobicity and complement C3 was detected bound to their surface. Antisera raised against a K-O- strain agglutinated the K+ strains grown in the presence but not in the absence of cefuroxime or ciprofloxacin. These findings suggest that the filamentous morphology induced by these antibiotics influences the distribution or amount of capsular polysaccharide such that cell envelope components previously masked by the capsule become accessible to complement and immunoglobulins.
在含有低于最低抑菌浓度（sub - MIC）的头孢呋辛或环丙沙星的培养基中，包膜化（K +）和非包膜化（K -）肺炎克雷伯菌菌株的生长导致细胞伸长，但对外膜蛋白或脂多糖谱影响很小。暴露于血清补体增加了K -菌株的表面疏水性，但未能与K +菌株相互作用或增加其表面疏水性。然而，在K +菌株在低于最低抑菌浓度的抗生素中生长后，补体增加了它们的表面疏水性，并且检测到补体C3结合在它们的表面。针对K - O -菌株产生的抗血清凝集在有头孢呋辛或环丙沙星存在而非不存在时生长的K +菌株。这些发现表明，这些抗生素诱导的丝状形态影响荚膜多糖的分布或数量，使得先前被荚膜掩盖的细胞包膜成分能够被补体和免疫球蛋白识别。
