Honda K, Ishiko O, Tatsuta I, Deguchi M, Hirai K, Nakata S, Sumi T, Yasui T, Ogita S
Department of Obstetrics and Gynecology, Osaka City University Medical School, Japan.
Cancer Res. 1995 Aug 15;55(16):3623-8.
Patients with advanced malignant neoplasms develop anemia and immunosuppression. During an attempt to identify the causes, we have found that plasma from such patients makes RBCs more fragile in hypotonic buffer, according to results obtained with a coil planet centrifuge. Plasma from these patients suppresses mitogen-stimulated lymphocyte proliferation. In this study, we identified the substance with these effects as a protein. During two-dimensional gel electrophoresis, two isomers with M(r) 50,000 and slightly different isoelectric points near 6.0 were found. Cell fractionation showed that these proteins were in both the cytosol and the nuclear fraction of cells in neoplasms. Another protein with the same antigenicity and a M(r) 100,000 found in the nuclear fraction of cells in neoplasms.
晚期恶性肿瘤患者会出现贫血和免疫抑制。在试图确定病因的过程中，根据使用盘管行星离心机获得的结果，我们发现此类患者的血浆会使红细胞在低渗缓冲液中更易破裂。这些患者的血浆会抑制有丝分裂原刺激的淋巴细胞增殖。在本研究中，我们确定具有这些作用的物质是一种蛋白质。在二维凝胶电泳中，发现了两种分子量为50,000且等电点在6.0附近略有不同的异构体。细胞分级分离显示，这些蛋白质存在于肿瘤细胞的胞质溶胶和核部分中。在肿瘤细胞的核部分中还发现了另一种具有相同抗原性且分子量为100,000的蛋白质。
