Ye J, Nishimura F, Orman R, Terranova V P
Laboratory of Tumor Biology and Connective Tissue Research, Columbia University, New York, New York 10032, USA.
J Dent Res. 1995 Jun;74(6):1303-9. doi: 10.1177/00220345950740061001.
Periodontal ligament (PDL) cells are believed to play a critically important role in the regeneration of the periodontium. We have suggested that polypeptide growth factors can enhance periodontal regeneration by stimulating PDL cell chemotaxis and mitogenesis. This manuscript describes the identification of a novel chemotactic factor isolated from human PDL cells which we named PDL-CTX. PDL-CTX induces the directed migration of human PDL cells in vitro and was found to be a more potent chemotactic agent than other known growth factors. Additionally, PDL-CTX has no chemotactic effect on gingival fibroblasts or gingival epithelial cells. Both tryptic digestion and boiling abolished PDL-CTX's biological activity. The designed purification method included Mono-S cation exchange, heparin-sepharose affinity, and microbore reverse-phase HPLC. The purified factor has a relative molecular weight of approximately 7000 daltons based on sodium dodecyl sulfate (SDS) gel analysis. The amino acid composition and partial amino acid sequence were determined from HPLC-purified material. These were determined to be unique. Further investigation of the biological functions of PDL-CTX on PDL cells and other ligament cells should help improve our understanding of ligament repair.
牙周韧带（PDL）细胞被认为在牙周组织再生中起着至关重要的作用。我们已经提出，多肽生长因子可以通过刺激PDL细胞趋化性和有丝分裂来增强牙周组织再生。本手稿描述了一种从人PDL细胞中分离出的新型趋化因子的鉴定，我们将其命名为PDL-CTX。PDL-CTX在体外诱导人PDL细胞的定向迁移，并且被发现是一种比其他已知生长因子更有效的趋化剂。此外，PDL-CTX对牙龈成纤维细胞或牙龈上皮细胞没有趋化作用。胰蛋白酶消化和煮沸均消除了PDL-CTX的生物活性。设计的纯化方法包括Mono-S阳离子交换、肝素-琼脂糖亲和层析和微孔反相高效液相色谱。根据十二烷基硫酸钠（SDS）凝胶分析，纯化后的因子相对分子量约为7000道尔顿。从高效液相色谱纯化的材料中测定了氨基酸组成和部分氨基酸序列。这些被确定是独特的。进一步研究PDL-CTX对PDL细胞和其他韧带细胞的生物学功能，应有助于增进我们对韧带修复的理解。
