菌毛-Opa+淋球菌在体外对上皮细胞的黏附涉及硫酸乙酰肝素。
Adherence of pilus- Opa+ gonococci to epithelial cells in vitro involves heparan sulfate.
作者信息
Chen T, Belland R J, Wilson J, Swanson J
机构信息
Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA.
出版信息
J Exp Med. 1995 Aug 1;182(2):511-7. doi: 10.1084/jem.182.2.511.
Neisseria gonorrhoeae attaches to host epithelial cells via pili and opacity-associated (Opa) outer membrane proteins. Pilus- gonococci (Gc) of strain MS11 adhere to both human and nonhuman cells, but only when particular Opa proteins are expressed; OpaA+ variants adhere best, OpaC+ variants are next best, and the seven other Opa+ variants adhere poorly or not at all. The adherence of OpaA+ Gc to Chinese hamster ovary (CHO) cells is inhibited by heparin or heparan sulfate (HS), but not by chondroitin sulfate. OpaA+ Gc do not adhere to CHO cells devoid of HS proteoglycans; low concentrations of heparin restore OpaA+ Gc adherence to these HS-deficient CHO cells and high concentrations inhibit it. 3H-heparin binding to whole Gc parallels their adherence abilities (OpaA+ > OpaC+ > OpaH+ >> Opas B, D, E, F, G, I = Opa- = 0). Opa proteins separated by SDS-PAGE also bind 3H-heparin. These data suggest that adherence of pilus-, Opa+ Gc involves HS-proteoglycan of eukaryotic cells.
淋病奈瑟菌通过菌毛和不透明相关（Opa）外膜蛋白附着于宿主上皮细胞。MS11菌株的菌毛淋病奈瑟菌（Gc）能附着于人和非人类细胞，但只有在表达特定Opa蛋白时才会发生；OpaA+变体附着能力最佳，OpaC+变体次之，其他七种Opa+变体附着能力较差或根本不附着。肝素或硫酸乙酰肝素（HS）可抑制OpaA+ Gc对中国仓鼠卵巢（CHO）细胞的附着，但硫酸软骨素无此作用。缺乏HS蛋白聚糖的CHO细胞上，OpaA+ Gc无法附着；低浓度肝素可恢复OpaA+ Gc对这些HS缺陷CHO细胞的附着能力，高浓度肝素则起抑制作用。3H-肝素与完整Gc的结合情况与其附着能力平行（OpaA+ > OpaC+ > OpaH+ >> Opas B、D、E、F、G、I = Opa- = 0）。经SDS-PAGE分离的Opa蛋白也能结合3H-肝素。这些数据表明，菌毛、Opa+ Gc的附着涉及真核细胞的HS蛋白聚糖。
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