Barbacid M, Robbins K C, Hino S, Aaronson S A
Proc Natl Acad Sci U S A. 1978 Feb;75(2):923-7. doi: 10.1073/pnas.75.2.923.
A strategy based on the identification of type-specific antigenic determinants in the transitional products of gag (p15, p12, and p30 proteins), pol (reverse transcriptase), and env (gp70 glycoproteins) genes of mammalian type C viruses has been used to study genetic recombination between these RNA viruses. By this approach, recombinants involving exogenous and endogenous mouse type C viruses have been identified and genetically mapped. Analogous techniques have been applied to investigate the genetic relationships between different classes of endogenous virus that exist within the same mouse cells. Proteins of the inducible class of xenotropic virus were shown to exhibit extensive antigenic homology with the gag but not the env gene products of the ecotropic virus class. Instead, the env gene-coded glycoproteins of the inducible and noninducible xenotropic virus classes possessed striking antigenic relatedness. These results, as well as supporting findings from molecular hybridization, favor the concept that the inducible xenotropic virus of mouse cells arose by a recombinational mechanism involving the progenitors of the other two endogenous virus classes.
一种基于识别哺乳动物C型病毒gag（p15、p12和p30蛋白）、pol（逆转录酶）和env（gp70糖蛋白）基因过渡产物中特定类型抗原决定簇的策略，已被用于研究这些RNA病毒之间的基因重组。通过这种方法，已鉴定出涉及外源性和内源性小鼠C型病毒的重组体并进行了基因定位。类似的技术已被应用于研究存在于同一小鼠细胞内不同类别的内源性病毒之间的遗传关系。已证明诱生性嗜异性病毒类的蛋白质与嗜亲性病毒类的gag基因产物表现出广泛的抗原同源性，但与env基因产物没有同源性。相反，诱生性和非诱生性嗜异性病毒类的env基因编码糖蛋白具有显著的抗原相关性。这些结果以及分子杂交的支持性发现，支持了这样一种概念，即小鼠细胞的诱生性嗜异性病毒是通过涉及其他两类内源性病毒祖细胞的重组机制产生的。
