Oehmichen M, Cröpelin A
Institute of Forensic Medicine, Medical University in Lübeck, Germany.
Int J Legal Med. 1995;107(5):257-62. doi: 10.1007/BF01245484.
The temporal course of epidermal basal cell proliferation in the wound of the pinna of rats was studied using bromodeoxyuridine (BrdU) immunohistochemistry. Following incisional wounding, the animals were sacrificed at intervals ranging from 0 hours to 32 days. Two biopsies were taken from each animal, one intravitally and one postmortem after 24 hours storage at 8 degrees C. Specimens were incubated in a solution containing BrdU and embedded in paraffin. BrdU expression was demonstrated by a monoclonal antibody against BrdU. In both intravital and postmortem biopsies, the labelling indices increased significantly in the period from 32 to 60 hours post-injury. This suggests that DNS synthesis induced during life continues after death. Applied to forensic practice, the present findings point to the possibility of determining the vitality of a wound in postmortem tissue.
采用溴脱氧尿苷（BrdU）免疫组织化学方法研究了大鼠耳廓伤口表皮基底细胞增殖的时间进程。切开伤口后，在0小时至32天的不同时间间隔处死动物。从每只动物身上取两块活检组织，一块是活体取样，另一块是在8℃储存24小时后死后取样。标本在含有BrdU的溶液中孵育，然后石蜡包埋。用抗BrdU单克隆抗体检测BrdU表达。在活体和死后活检中，损伤后32至60小时标记指数均显著增加。这表明生前诱导的DNA合成在死后仍在继续。应用于法医实践，目前的研究结果表明在死后组织中确定伤口活力的可能性。
