Massa M, De Benedetti F, Pignatti P, Albani S, Monestier M, Martini A
Università degli Studi di Pavia, IRCCS Policlinico San Matteo, Italy.
Br J Rheumatol. 1995 Jun;34(6):507-11. doi: 10.1093/rheumatology/34.6.507.
This study aimed to measure IgG reactivities to DNA-free nucleosome subparticles (H2AH2B, H3H4) and nucleosome subparticles (H2AH2B-DNA, H3H4-DNA) and to evaluate the temporal relation of these reactivities, as well as those to single core histones, with iridocyclitis (IC) in patients with antinuclear antibody positive (ANA+) pauciarticular juvenile chronic arthritis (JCA). Reactivities to nuclear substrates were determined by enzymatic immunoassays in 120 sera from 45 children with ANA+ pauciarticular JCA. Significantly elevated IgG levels to H3 and H4, to DNA-free nucleosome subparticles, and to the nucleosome subparticle H3H4-DNA were present in patients with ANA+ pauciarticular JCA; no evidence of recognition of conformational epitopes was found. In both horizontal and follow-up studies, no relation between the reactivities studied and the development, presence, or history of IC was found. Our results show the absence of a relation of antibodies to histone molecules or to nucleosome subparticles with IC in patients with ANA+ pauciarticular JCA.
本研究旨在检测抗核抗体阳性（ANA+）少关节型幼年慢性关节炎（JCA）患者血清中针对无DNA核小体亚颗粒（H2AH2B、H3H4）和核小体亚颗粒（H2AH2B-DNA、H3H4-DNA）的IgG反应性，并评估这些反应性以及针对单个核心组蛋白的反应性与虹膜睫状体炎（IC）之间的时间关系。通过酶免疫测定法检测了45例ANA+少关节型JCA患儿的120份血清对核底物的反应性。ANA+少关节型JCA患者血清中针对H3和H4、无DNA核小体亚颗粒以及核小体亚颗粒H3H4-DNA的IgG水平显著升高；未发现识别构象表位的证据。在横向研究和随访研究中，均未发现所研究的反应性与IC的发生、存在或病史之间存在关联。我们的结果表明，ANA+少关节型JCA患者血清中针对组蛋白分子或核小体亚颗粒的抗体与IC之间不存在关联。
