Egeskjold E M, Johansen A, Permin H, Høyeraal H M, Sørensen T
Acta Paediatr Scand. 1982 Jul;71(4):615-20. doi: 10.1111/j.1651-2227.1982.tb09484.x.
Serum samples from 8 children with juvenile rheumatoid arthritis (JRA) and chronic bilateral iridocyclitis were significantly distinguished from 5 children with JRA and no eye symptoms by the presence of large immune complexes (IC) greater than 22S, IgM antinuclear antibodies (ANA), IgG granulocyte-specific (GS-) ANA, C3 fixing ANA, and IgM anti-IgG. One serum with and two sera without IC greater than 22S, all from patients with iridocyclitis, were fractionated by rate zonal ultracentrifugation. Each fraction relevant for the study was separately concentrated and reexamined. In one of the sera without IC greater than 22S this technique exposed the presence of IgA GS-ANA not detectable in the corresponding whole serum. IgG ANA were precipitated in an area with higher molecular weight than the one for IgG indicating the presence of aggregated IgG ANA. Fractionation of the serum with IC greater than 22S demonstrated IgM GS-ANA not present in whole serum. The results support previous suggestions that ANA may be involved in the pathogenesis of chronic iridocyclitis and may explain why ANA (in particular C3 fixing ANA) negative patients with JRA rarely develop chronic iridocyclitis.
来自8名患有青少年类风湿性关节炎（JRA）和慢性双侧虹膜睫状体炎的儿童的血清样本，与5名患有JRA但无眼部症状的儿童的血清样本有显著区别，区别在于存在大于22S的大免疫复合物（IC）、IgM抗核抗体（ANA）、IgG粒细胞特异性（GS-）ANA、C3固定ANA和IgM抗IgG。对1份有大于22S的IC的血清和2份无大于22S的IC的血清（均来自虹膜睫状体炎患者）进行了速率区带超速离心分离。与研究相关的每个组分分别进行浓缩并重新检测。在1份无大于22S的IC的血清中，该技术显示出在相应的全血清中未检测到的IgA GS-ANA的存在。IgG ANA沉淀在分子量高于IgG的区域，表明存在聚集的IgG ANA。对有大于22S的IC的血清进行分离显示全血清中不存在IgM GS-ANA。这些结果支持了先前的推测，即ANA可能参与慢性虹膜睫状体炎的发病机制，并且可以解释为什么JRA中ANA（特别是C3固定ANA）阴性的患者很少发生慢性虹膜睫状体炎。
